产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12197, RRID:AB_2845002.
展开/折叠
AN; AUNA1; Dia2; diap3; DIAP3_HUMAN; DIAPH3; Diaphanous homolog 3 (Drosophila); Diaphanous homolog 3; Diaphanous related formin 3; Diaphanous, Drosophila, homolog of, 3; Diaphanous-related formin-3; DKFZp434C0931; DKFZp686A13178; DRF3; FLJ34705; mDia2; NSDAN; OTTHUMP00000018480; Protein diaphanous homolog 3; RP11-26P21.1;
抗原和靶标
- Q9NSV4 DIAP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERHQPRLHHPAQGSAAGTPYPSSASLRGCRESKMPRRKGPQHPPPPSGPEEPGEKRPKFHLNIRTLTDDMLDKFASIRIPGSKKERPPLPNLKTAFASSDCSAAPLEMMENFPKPLSENELLELFEKMMEDMNLNEDKKAPLREKDFSIKKEMVMQYINTASKTGSLKRSRQISPQEFIHELKMGSADERLVTCLESLRVSLTSNPVSWVESFGHEGLGLLLDILEKLISGKIQEKVVKKNQHKVIQCLKALMNTQYGLERIMSEERSLSLLAKAVDPRHPNMMTDVVKLLSAVCIVGEESILEEVLEALTSAGEEKKIDRFFCIVEGLRHNSVQLQVACMQLINALVTSPDDLDFRLHIRNEFMRCGLKEILPNLKCIKNDGLDIQLKVFDEHKEEDLFELSHRLEDIRAELDEAYDVYNMVWSTVKETRAEGYFISILQHLLLIRNDYFIRQQYFKLIDECVSQIVLHRDGMDPDFTYRKRLDLDLTQFVDICIDQAKLEEFEEKASELYKKFEKEFTDHQETQAELQKKEAKINELQAELQAFKSQFGALPADCNIPLPPSKEGGTGHSALPPPPPLPSGGGVPPPPPPPPPPPLPGMRMPFSGPVPPPPPLGFLGGQNSPPLPILPFGLKPKKEFKPEISMRRLNWLKIRPHEMTENCFWIKVNENKYENVDLLCKLENTFCCQQKERREEEDIEEKKSIKKKIKELKFLDSKIAQNLSIFLSSFRVPYEEIRMMILEVDETRLAESMIQNLIKHLPDQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEKYPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKVCNHENQKVQLTEGSRSHYNINCNSTRTPVAKELNYNLDTHTSTGRIKAAEKKEACNVESNRKKETELLGSFSKNESVPEVEALLARLRAL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NSV4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R7 | Methylation | Uniprot | |
| T19 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S23 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| R28 | Methylation | Uniprot | |
| R31 | Methylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K94 | Methylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| K518 | Ubiquitination | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| K536 | Ubiquitination | Uniprot | |
| K548 | Ubiquitination | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| K653 | Methylation | Uniprot | |
| K672 | Ubiquitination | Uniprot | |
| Y673 | Phosphorylation | Uniprot | |
| K681 | Ubiquitination | Uniprot | |
| K691 | Ubiquitination | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| S717 | Phosphorylation | Uniprot | |
| K718 | Ubiquitination | Uniprot | |
| S728 | Phosphorylation | Uniprot | |
| S729 | Phosphorylation | Uniprot | |
| K759 | Ubiquitination | Uniprot | |
| K874 | Ubiquitination | Uniprot | |
| K909 | Ubiquitination | Uniprot | |
| K912 | Ubiquitination | Uniprot | |
| K920 | Ubiquitination | Uniprot | |
| K934 | Ubiquitination | Uniprot | |
| K948 | Ubiquitination | Uniprot | |
| K961 | Ubiquitination | Uniprot | |
| K991 | Ubiquitination | Uniprot | |
| K1032 | Ubiquitination | Uniprot | |
| K1053 | Ubiquitination | Uniprot | |
| S1091 | Phosphorylation | Uniprot | |
| S1093 | Phosphorylation | Uniprot | |
| S1096 | Phosphorylation | Uniprot | |
| K1110 | Ubiquitination | Uniprot | |
| K1134 | Ubiquitination | Uniprot | |
| Y1138 | Phosphorylation | Uniprot | |
| S1145 | Phosphorylation | Uniprot | |
| S1162 | Phosphorylation | Uniprot | |
| K1166 | Ubiquitination | Uniprot | |
| K1176 | Ubiquitination | Uniprot | |
| S1179 | Phosphorylation | Uniprot |
研究背景
Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.
Ubiquitinated.
Cytoplasm. Nucleus.
Note: During mitosis, co-localizes with the actin-rich cleavage furrow and with the microtubule-rich central spindle during cytokinesis (PubMed:18755006, PubMed:19457867). Shuttles between the cytoplasm and the nucleus (By similarity).
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.
Belongs to the formin homology family. Diaphanous subfamily.
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
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