Collagen Type VI Antibody - #DF12191

Fig. 9 Integrative analysis of proteomics and metabolomics. A Venn diagram showed three pathways co-enriched in proteomics and metabolomics, including Protein digestion and absorption, PI3K-Akt signaling pathway, and FoxO signaling pathway. B Interaction network diagram of differential proteins and metabolites among the three pathways. C Western blot verified that DPP4, CCND1, CDK2, and FGF2 were up-regulated and COL6A1 and SOD2 were down-regulated in the TGF-β1-treated MRC-5 cells and BLM-induced mice. D l-Glutamine, l-proline, and AMP increased, and l-arginine, l-phenylalanine, l-lysine, and l-tryptophan decreased in the TGF-β1-treated MRC-5 cells and BLM-induced mice. E Western blot demonstrated that circ0066187 knockdown decreased DPP4, CCND1, CDK2, and FGF2 expression and increased COL6A1 and SOD2 expression compared with those in TGF-β1-treated group. Circ0066187 overexpression enhanced DPP4, CCND1, CDK2, and FGF2 expression, and decreased COL6A1 and SOD2 expression. F miR-29b-2-5p mimic enhanced COL6A1 and SOD2 expression and inhibited DPP4, CCND1, CDK2, and FGF2 expression. miR-29b-2-5p inhibitor enhanced DPP4, CCND1, CDK2, and FGF2 expression and inhibited COL6A1 and SOD2 expression. G Rescue experiment of Western blot demonstrated that the miR-29b-2-5p inhibitor reversed the downward trend of DPP4, CCND1, CDK2, and FGF2 and the upward trend of COL6A1 and SOD2 caused by si-circ0066187. miR-29b-2-5p mimic reversed the upward trend of DPP4, CCND1, CDK2, and FGF2 and the downward trend of COL6A1 and SOD2 caused by circ0066187 overexpression. H Western blot showed that si-STAT3 decreased DPP4, CCND1, CDK2, and FGF2 expression, and increased COL6A1 and SOD2 expression. Overexpressed STAT3 enhanced DPP4, CCND1, CDK2, and FGF2 expression and inhibited COL6A1 and SOD2 expression. I Rescue experiment showed that STAT3 overexpression reversed the downward trend of DPP4, CCND1, CDK2, and FGF2 and the upward trend of COL6A1 and SOD2 caused by si-circ0066187. si-STAT3 reversed the upward trend of DPP4, CCND1, CDK2, and FGF2 and the downward trend of COL6A1 and SOD2 caused by circ0066187 overexpression. NC represents negative control, BP represents blank plasmid, and RP represents the recombinant plasmid of overexpressed circ0066187. OE-STAT3 represents overexpressed STAT3. Each bar represents the mean ± SD; n = 6; *p