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| 产品: | TTLL6 抗体 |
| 货号: | DF12178 |
| 描述: | Rabbit polyclonal antibody to TTLL6 |
| 应用: | WB IHC |
| 文献验证: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| 蛋白号: | Q8N841 |
| RRID: | AB_2844983 |
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TTL Antibody detects endogenous levels of total TTLL6.
RRID:
AB_2844983
引用格式: Affinity Biosciences Cat# DF12178, RRID:AB_2844983.
引用格式: Affinity Biosciences Cat# DF12178, RRID:AB_2844983.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
FLJ35808; TTL 6; TTL.6; Tubulin polyglutamylase TTLL6; Tubulin tyrosine ligase like family, member 6;
抗原和靶标
免疫原:
A synthesized peptide derived from human TTLL6, corresponding to a region within N-terminal amino acids.
基因/基因ID:
文献引用
1). Repeated Exposure to Sevoflurane in Neonatal Mice Induces Cognitive and Synaptic Impairments in a TTLL6-Mediated Tubulin Polyglutamylation Manner. CNS neuroscience & therapeutics, 2025
(PubMed: 40202105)
[IF=4.8]
Application: WB Species: Mouse Sample:
FIGURE 3 Effects of repeated sevoflurane exposure on Tau and TTLL6 missorting in hippocampus of neonatal mice. (A) Expression of polyglutamylated Tubulin and TTLL6 in the hippocampus after control or repetitive sevoflurane treatment. (B) Qualification of polyglutamylated tubulin/TTLL6 in different groups; n = 6 mice/group. (C) Expression of AT8 and Tau5 in the hippocampus after control or repetitive sevoflurane treatment. (D) Qualification of AT8/Tau5 in different groups; n = 6 mice/group. (E) Results of microtubule binding assay in the hippocampus of neonatal mice in Control and Sevoflurane groups, (F) the supernatanted protein representing microtubule-unbound Tau, (G) the pelleted protein representing microtubule-bond Tau; n = 3 mice/group. (H) Immunoprecipitation analysis of Tau5 and TTLL6 interaction in hippocampus cells. IgG: Negative control; Input: Total protein and positive control; IB: Immunoblotting; IP: Immunoprecipitation; n = 3 biological replicates. (I) Immunofluorescence of the protein labeled by pTau (red) and TTLL6 (green) in the CA3 area of hippocampus after control or repetitive sevoflurane treatment, repeated sevoflurane treatment increased pTau-TTLL6 co-expressed levels (yellow) in cell body and dendrites (marked with write arrows); blue is DAPI, scale bar: 50 μm. Qualification of (J) pTau and (K) TTLL6 fluorescence intensity in SR area of CA3 region in different groups; n = 3 biological replicates. SR, Stratum radiatum area: Composed mostly of apical dendrites of pyramidal neurons; SP: Stratum pyramidale: Composed mostly of tightly arranged pyramidal cells. *p
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