TRIM44 Antibody - #DF12177

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产品: TRIM44 抗体
货号: DF12177
描述: Rabbit polyclonal antibody to TRIM44
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q96DX7
RRID: AB_2844982

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TRIM44 Antibody detects endogenous levels of total TRIM44.
RRID:
AB_2844982
引用格式: Affinity Biosciences Cat# DF12177, RRID:AB_2844982.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AN3; DIPB; HSA249128; MC7; MGC3490; Protein DIPB; TRI44_HUMAN; TRIM44; Tripartite motif containing 44; Tripartite motif containing protein 44; Tripartite motif-containing protein 44;

抗原和靶标

免疫原:

A synthesized peptide derived from human TRIM44, corresponding to a region within the internal amino acids.

基因/基因ID:
TRIM44(54765)

文献引用

1). Long noncoding RNA KCNQ1OT1 promotes proliferation, migration, and invasion via targeting miR‐134 in retinoblastoma. JOURNAL OF GENE MEDICINE, 2021 (PubMed: 33818859) [IF=3.2]

Application: WB    Species: Human    Sample: Retinoblastoma (Rb) tissues

FIGURE 5 TRIM44 is a molecular target of miR-134. (A) Schematic of TRIM44 30 -UTR wild-type (WT) and mutant (Mut) luciferase reporter vectors. (B) Verification of TRIM44 as a target gene of miR-134 by the dual-luciferase reporter assay. (C) Detection of TRIM44 mRNA expression in Weri-Rb1, Y79 and ARPE-19. (D) Detection of TRIM44 mRNA-expressing levels in Rb tissues and adjacent normal tissues (n = 50). (E) Linear regression analysis for the correlation between TRIM44 and miR-134 in Rb tissues (n = 50). (F) Detection of TRIM44 protein expression in WeriRb1, Y79, and ARPE-19. (G) Detection of TRIM44 protein expression in Rb and adjacent normal tissues (n = 50). (H) Analysis of TRIM44 protein expression in Weri-Rb1 and Y79 cells transfected with miR-NC or miR-134. Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01

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