MCM10 Antibody - #DF12162

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产品: MCM10 抗体
货号: DF12162
描述: Rabbit polyclonal antibody to MCM10
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: Q7L590
RRID: AB_2844967

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
MCM10 Antibody detects endogenous levels of total MCM10.
RRID:
AB_2844967
引用格式: Affinity Biosciences Cat# DF12162, RRID:AB_2844967.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CNA43; DNA43; FLJ31133; Homolog of yeast MCM10; HsMCM10; mcm10; MCM10 minichromosome maintenance deficient 10; MCM10_HUMAN; Minichromosome maintenance 10 replication initiation factor; Minichromosome maintenance complex component 10; Minichromosome maintenance protein 10 isoform 1; PRO2249; Protein MCM10 homolog;

抗原和靶标

免疫原:

A synthesized peptide derived from human MCM10, corresponding to a region within C-terminal amino acids.

基因/基因ID:
MCM10(55388)

文献引用

1). Minichromosome maintenance protein 10 as a marker for proliferation and prognosis in lung cancer. International Journal of Oncology, 2019 (PubMed: 31638210) [IF=4.5]

Application: WB    Species: Human    Sample: A549 cells

Figure 7 - Downregulation of MCM10 induces G1 arrest by suppressing CCND1 expression in vitro. (A) Flow cytometry analysis of cell-cycle in A549 and H661 cells with or without MCM10 depletion. (B) Reverse transcription-quantitative PCR was performed to explore the expression of CDK1, CDK2, CDK4, CDK6, P27, P19, P21, CCNA2, CCND1, CCNB1, CCNE1 in siMCM10 and NC groups of A549 cells. (C) Relative expression of CCND1 after MCM10 knockdown in A549 and H661 cells. (D) Western blotting assay showing MCM10 and CCND1 protein expression level after MCM10 knockdown in A549 and H661 cells. GAPDH was used as a loading control. Data are presented as the mean ± SEM of three independent experiments, each performed in triplicate. *P

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