ACSL4/FACL4 Antibody - #DF12141

Fig. 2 PS-NPs induce testicular ferroptosis in mice. (A-C) The concentration of total Fe, MDA and GSH in mice serum after PS-NPs exposure. (D-F) Effect of PS-NPs on total Fe, MDA and GSH in mice testes. (G) Gene expression of ferroptosis-associated genes in isolated testes using qRT-PCR after treatment. (H and I) Western blotting and quantitative analysis of ferroptosis-related protein expression. (J and K) Immunofluorescence images of GPX4 and SLC7A11 in testes following PS-NPs treatment for 35 days

Fig. 1. Renal tubular epithelial cells undergo ferroptosis in I/R-AKI. (A) Single-cell sequencing identified clusters in both sham-operated and I/R-operated kidneys at day 3 post-surgery, depicted in a t-distributed stochastic neighbor embedding (tSNE) map. PT, proximal tubule; DT, distal convoluted tubule; LOH, ascending loop of Henle; ECs, endothelial cells. (B) Average expression levels of mouse single cell type-specific genes are shown in pheatmap. Mean expression values of the genes were calculated in each cluster. The full list of cell types and genes is shown in Additional file1: Table S1. (C-E) Subtypes of PT with differentially expressed genes are illustrated in a tSNE plot (C), and the representative marker genes across the 3 subtypes of PT are depicted in pheatmap (D) and VlnPlot (E). (F-G) The expression levels of ferroptosis-associated genes Gpx4 and Acsl4 in the subtypes of PT were identified by FeaturePlot (F) and Dotplot (G). (H-I) Western blot (H) and statistical analysis (I) of the expression levels of GPX4 and SLC7A11 proteins in the renal cortex of sham-operated and I/R-operated mice post-surgery. ***p