ACSL4/FACL4 Antibody - #DF12141

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产品: ACSL4/FACL4 抗体
货号: DF12141
描述: Rabbit polyclonal antibody to ACSL4/FACL4
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白ID: O60488
RRID: AB_2844946

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#DF12141-BP

ACSL4/FACL4 Peptide

1mg
相关下载
抗原肽/阻断肽MS报告
抗原肽/阻断肽HPLC报告
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ACSL4/FACL4 Antibody detects endogenous levels of total ACSL4/FACL4.
RRID:
AB_2844946
引用格式: Affinity Biosciences Cat# DF12141, RRID:AB_2844946.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACS 4; ACS4; ACSL 4; Acsl4; ACSL4_HUMAN; acyl CoA synthetase 4; Acyl CoA synthetase long chain family member 4; FACL 4; FACL4; Fatty acid Coenzyme A ligase; fatty acid Coenzyme A ligase long-chain 4; LACS 4; LACS4; Lignoceroyl CoA synthase; Long chain 4; long chain acyl CoA synthetase 4; long chain fatty acid CoA ligase 4; long chain fatty acid Coenzyme A ligase 4; Long-chain acyl-CoA synthetase 4; Long-chain-fatty-acid--CoA ligase 4; MRX63; MRX68;

抗原和靶标

免疫原:

A synthesized peptide derived from human ACSL4/FACL4, corresponding to a region within N-terminal amino acids.

基因/基因ID:
ACSL4(2182)

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Pathology-Responsive Self-Assembling Hydrogel Enabling Spatiotemporally Controlled Exosome Release and Redox Regulation for Intervertebral Disc Regeneration. ADVANCED MATERIALS, 2026 [IF=29.1]
2). Glycolysis-Derived Lactate Induces ACSL4 Expression and Lactylation to Activate Ferroptosis during Intervertebral Disc Degeneration. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 40171826) [IF=15.1]
3). Identification and analysis of microplastic aggregation in CAR-T cells. Journal of hazardous materials, 2024 (PubMed: 39488976) [IF=13.6]
4). Sonocatalytic Eradication of Hepatocellular Carcinoma by Tailoring Structural Defects of Black Indium Oxide Sonocatalysts and Leveraging Apoptosis/Ferroptosis-Hybridized Pathways. Small (Weinheim an der Bergstrasse, Germany), 2026 (PubMed: 41627202) [IF=13.0]
5). Time-restricted feeding protects against septic liver injury by reshaping gut microbiota and metabolite 3-hydroxybutyrate. Gut microbes, 2025 (PubMed: 40223164) [IF=12.2]

Application: IF/ICC    Species: Mouse    Sample: liver tissue

Figure 6. 3-HB inhibits ferroptosis in mice by activating the PI3K/AKT/mTOR/LPIN1 pathway. (a) GSEA-kegg analysis. (b) Relative mRNA levels of Acsl4, Gpx4, SLC7a11, Fth1 and hmox-1 genes, n = 5–6. (c, d) ACSL4 immunofluorescence and quantification in liver tissues, scale: 100 μm, n = 6. (e-g) MDA, LPO and Fe2+ content in liver tissue, n = 6. (h, i) ACSL4 immunofluorescence and quantification in liver tissues, scale: 100 μm, n = 6. (j) Relative ACSL4 mRNA expression level, n = 6–8. (k-m) MDA, LPO and Fe2+ content in liver tissue from mice in the CLP, CLP+NVP-BEZ235, CLP + 3-HB, and CLP + 3-HB+NVP-BEZ235 groups, n = 6. (n) ACSL4 immunofluorescence of liver tissue from WT and Lpin1KO mice, scale: 100 μm, n = 6. (o) Quantitative analysis of ACSL4 immunofluorescence intensity, n = 6. (p) Relative ACSL4 mRNA expression level, n = 6–8. (q-s) MDA, LPO and Fe2+ content in liver tissue from WT and Lpin1KO mice, n = 6. The results are expressed as the median and quartile. * p 

Application: WB    Species: Mouse    Sample: AML12 cells

Figure 7. 3-HB alleviates AML12 cell injury by activating the PI3K/AKT/mTOR/LPIN1 pathway to inhibit ferroptosis. (a) AML12 cell viability was detected by CCk8, n = 6. (b) LDH content in AML12 cell supernatant, n = 6. (c-f) MDA, LPO, TGSH and Fe2+ content in the AML12 cells, n = 4. (g) AML12 cell viability by CCk8, n = 6. (h) LDH content in AML12 cell supernatant, n = 6. (i-k) the protein expression levels of p-akt, PI3K, p-mTOR, LPIN1 and ACSL4 in the AML12 cells treated with LPS, LPS+NVP-BEZ235, LPS + 3-HB, and LPS + 3-HB+NVP-BEZ235 (n = 3). (l-o) MDA, LPO, TGSH and Fe2+ content in the AML12 cells treated with LPS, LPS+NVP-BEZ235, LPS + 3-HB, and LPS + 3-HB+NVP-BEZ235, n = 4. (p) LPIN1 protein expression level, n = 4. (q) The protein expression levels of LPIN1 and ACSL4 in the AML12 cells (n = 3). (r) AML12 cell viability by CCk8, n = 6. (s) LDH content in AML12 cell supernatant, n = 6. (t-w) MDA, LPO, TGSH and Fe2+ content in AML12 cells transfected with Ctrl shRNA and Lpin1 shRNA, n = 4. The results are expressed as the mean ± SEM. *p < 0.05，**p 
6). Interplay between lipid dysregulation and ferroptosis in chondrocytes and the targeted therapy effect of metformin on osteoarthritis. Journal of advanced research, 2025 (PubMed: 38621621) [IF=11.4]
7). The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3. Redox biology, 2023 (PubMed: 37099926) [IF=10.7]
8). Dual-responsive stem cell microspheres modified with BDNF for enhanced neural repair in diabetic erectile dysfunction. Journal of controlled release : official journal of the Controlled Release Society, 2025 (PubMed: 39761859) [IF=10.5]
9). Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte. Journal of nanobiotechnology, 2025 (PubMed: 40707955) [IF=10.2]

Application: WB    Species: Mouse    Sample:

Fig. 2 PS-NPs induce testicular ferroptosis in mice. (A-C) The concentration of total Fe, MDA and GSH in mice serum after PS-NPs exposure. (D-F) Effect of PS-NPs on total Fe, MDA and GSH in mice testes. (G) Gene expression of ferroptosis-associated genes in isolated testes using qRT-PCR after treatment. (H and I) Western blotting and quantitative analysis of ferroptosis-related protein expression. (J and K) Immunofluorescence images of GPX4 and SLC7A11 in testes following PS-NPs treatment for 35 days
10). EBF1-induced CSRP2 boosts the progression of B-cell acute lymphocytic leukemia by inhibiting ferroptosis. Cancer letters, 2025 (PubMed: 39952599) [IF=9.1]

Application: WB    Species: human    Sample:

Fig. 3. Knockdown of CSRP2 induced B-ALL ferroptosis. (A) The cellular Fe2+ levels after knockdown of CSRP2. (B) The cellular GSH levels after knockdown of CSRP2. (C) The cellular MDA levels after knockdown of CSRP2. (D) Representative image of cellular JC-1 staining for MMP measurement after knockdown of CSRP2. (E) Quantification of cellular MMP levels using ImageJ software. (F) The mRNA levels of SLC7A11, GPX4 and ACSL4 detected by qRT-PCR after knockdown of CSRP2. (G) The protein levels of SLC7A11, GPX4 and ACSL4 detected by Western blotting after knockdown of CSRP2. (H) The Western blotting results were quantified by ImageJ software. ∗P < 0.05, ∗∗P < 0.01. The experiment was repeated three times independently.
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