Phospho-Smad3 (Ser425) Antibody - #AF3362

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产品: 磷酸化 Smad3 (Ser425) 抗体
货号: AF3362
描述: Rabbit polyclonal antibody to Phospho-Smad3 (Ser425)
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P84022
RRID: AB_2834777

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 50ul RMB¥ 1300 现货
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Smad3 (Ser425) Antibody detects endogenous levels of Smad3 only when phosphorylated at Serine 425.
RRID:
AB_2834777
引用格式: Affinity Biosciences Cat# AF3362, RRID:AB_2834777.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZP586N0721; DKFZp686J10186; hMAD 3; hMAD-3; hSMAD3; HSPC193; HST17436; JV15 2; JV15-2; JV152; LDS1C; LDS3; MAD (mothers against decapentaplegic Drosophila) homolog 3; MAD homolog 3; Mad homolog JV15 2; Mad protein homolog; MAD, mothers against decapentaplegic homolog 3; Mad3; MADH 3; MADH3; MGC60396; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD family member 3; SMAD, mothers against DPP homolog 3; Smad3; SMAD3_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425.

基因/基因ID:
SMAD3(4088)
描述:
Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Rescuing ACE2-Deficiency-Mediated Nucleus Pulposus Senescence and Intervertebral Disc Degeneration by a Nanotopology-Enhanced RNAi System. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 39804951) [IF=15.1]
2). Enhancing BMSC chondrogenesis with a dynamic viscoelastic hyaluronan hydrogel loaded with kartogenin for cartilage repair. International journal of biological macromolecules, 2025 (PubMed: 40345284) [IF=7.7]

Application: WB    Species: rabbit    Sample: BMSCs

Fig. 7. Transcriptomic analysis of BMSC chondrogenesis in the RHAO@KGN and HAO groups. (A) Volcano map of significantly up-regulated (red) and down-regulated (blue) genes in different groups. (B) Heatmap of typical DEGs. (C) GO analysis for upregulated genes in BMSCs. (D) KEGG analysis for upregulated genes in BMSCs. (E) GSEA enrichment analysis of TGF-β signaling pathway in BMSCs. (F) WB analysis of the expression of the related proteins of the TGF-β/SMAD signaling pathway. (G) Schematic diagram of chondrogenesis through TGF-β signaling pathway in RHAO@KGN group.
3). Roscovitine attenuates renal interstitial fibrosis in diabetic mice through the TGF-β1/p38 MAPK pathway. BIOMEDICINE & PHARMACOTHERAPY, 2019 (PubMed: 31029000) [IF=6.9]

Application: WB    Species: human    Sample: HK2 cells

Fig. 5. |Effect of roscovitine on the expression of α-SMA, E-Cadherin, collagen I, TGF-β1, p-Smad3 and p-p38 MAPK in high glucose treated HK2. (A) Representative western blot showing α-SMA, E-Cadherin and collagen I. (B) Representative western blot showing TGF-β1, p-Smad3 and p-p38 MAPK. (C)-(H) Quantitative analyses of the results from western blot. The each protein level was normalized to β-actin. *P < 0.05, n=3.
4). L-Glutamine alleviates osteoarthritis by regulating lncRNA-NKILA expression through the TGF-β1/SMAD2/3 signalling pathway. Clinical Science, 2022 (PubMed: 35730575) [IF=6.7]
5). Overexpressed Poldip2 Incurs Retinal Fibrosis via the TGF-β1/SMAD3 Signaling Pathway in Diabetic Retinopathy. Diabetes, 2024 (PubMed: 38968428) [IF=6.2]
6). Lupiwighteone Ameliorates Diabetic Nephropathy-Induced Renal Fibrosis via SIRT1/NF-κB Signaling Pathway-Mediated Inflammatory Response. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2025 [IF=6.2]
7). Sodium arsenite induces hepatic stellate cells activation by m6A modification of TGF-β1 during liver fibrosis. Ecotoxicology and environmental safety, 2024 (PubMed: 38714084) [IF=6.2]
8). Timosaponin BII inhibits TGF‐β mediated epithelial‐mesenchymal transition through Smad‐dependent pathway during pulmonary fibrosis. PHYTOTHERAPY RESEARCH, 2023 (PubMed: 36807664) [IF=6.1]
9). Design, synthesis and evaluation of a pyrazolo[3,4-d]pyrimidine derivative as a novel and potent TGFβ1R1 inhibitor. European journal of medicinal chemistry, 2024 (PubMed: 38626523) [IF=6.0]
10). MBNL2 promotes aging-related cardiac fibrosis via inhibited SUMOylation of Krüppel-like factor4. iScience, 2024 (PubMed: 38974966) [IF=5.8]
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