TIP47 Antibody - #DF12139

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产品: TIP47 抗体
货号: DF12139
描述: Rabbit polyclonal antibody to TIP47
应用: WB IHC IF/ICC
文献验证: IF/ICC
反应: Human, Mouse, Rat
分子量: 47 kDa; 47kD(Calculated).
蛋白号: O60664
RRID: AB_2844944

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TIP47 Antibody detects endogenous levels of total TIP47.
RRID:
AB_2844944
引用格式: Affinity Biosciences Cat# DF12139, RRID:AB_2844944.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; M6PRBP 1; M6PRBP1; Mannose 6 phosphate receptor binding protein 1; Mannose-6-phosphate receptor-binding protein 1; MGC11117; MGC2012; MPR binding protein 47 kDa; Perilipin 3; Perilipin-3; Perilipin3; Placental protein 17; PLIN3; PLIN3_HUMAN; pp17; Tail interacting protein 47 kD; TIP47;

抗原和靶标

免疫原:
Uniprot:

O60664(PLIN3_HUMAN) >>Visit HPA database.

基因/基因ID:
PLIN3(10226)
序列:
MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHIKTVCDAAEKGVRTLTAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAKDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAELARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHSLGKLRATKQRAQEALLQLSQVLSLMETVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSSILAQSRERVASAREALDHMVEYVAQNTPVTWLVGPFAPGITEKAPEEKK

研究背景

功能:

Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.

细胞定位:

Cytoplasm. Endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lipid droplet.
Note: Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homooligomer. Interacts with M6PR (via the cytoplasmic domain). Interacts with IGF2R (via the cytoplasmic domain). Isoform 2 may exist as a homodimer (known as PP17C).

蛋白家族:

Belongs to the perilipin family.

文献引用

1). Interface-Targeting Strategy Enables Two-Photon Fluorescent Lipid Droplet Probes for High-Fidelity Imaging of Turbid Tissues and Detecting Fatty Liver. ACS Applied Materials & Interfaces, 2018 (PubMed: 29521495) [IF=8.3]

Application: IF/ICC    Species:    Sample: HeLa cells

Figure S24|Confocal fluorescence images of HeLa cells co@stained with N@Cy and anti@TIP47 antiserum. (1): DIC; (2): N-Cy; (4): Anti@TIP47 antiserum; (4): Merged image of (2) and (3); (5): Merged image of (1) @ (3). (6): The normalized fluorescence profiles of N-Cy and ER red along the white line in (4).N-Cy: 4 UM, 2 min; λex = 488 nm, λem = 510@540 nm. Anti@TIP47 antiserum (fluorescence from Alexa Fluor 594@conjugated goat anti@rabbit IgG): λex = 561 nm; λem = 600@700 nm. Bar = 20 Um.

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