产品: PPAT 抗体
货号: DF12131
描述: Rabbit polyclonal antibody to PPAT
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 50-56kd; 57kD(Calculated).
蛋白号: Q06203
RRID: AB_2844936

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
克隆:
Polyclonal
特异性:
PPAT Antibody detects endogenous levels of total PPAT.
RRID:
AB_2844936
引用格式: Affinity Biosciences Cat# DF12131, RRID:AB_2844936.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Amidophosphoribosyltransferase [Precursor]; Amidophosphoribosyltransferase; ATase; Glutamine phosphoribosylpyrophosphatate amidotransferase; Glutamine phosphoribosylpyrophosphate amidotransferase; Glutamine PRPP amidotransferase; GPAT; Phosphoribosyl pyrophosphate amidotransferase; Ppat; PRAT; PUR1_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q06203 PUR1_HUMAN:

Ubiquitously expressed.

序列:
MELEELGIREECGVFGCIASGEWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSSVPTFKSHKGMGLVNHVFTEDNLKKLYVSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRHGIGLSTSSDSEMITQLLAYTPPQEQDDTPDWVARIKNLMKEAPTAYSLLIMHRDVIYAVRDPYGNRPLCIGRLIPVSDINDKEKKTSETEGWVVSSESCSFLSIGARYYREVLPGEIVEISRHNVQTLDIISRSEGNPVAFCIFEYVYFARPDSMFEDQMVYTVRYRCGQQLAIEAPVDADLVSTVPESATPAALAYAGKCGLPYVEVLCKNRYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRIVLVDDSIVRGNTISPIIKLLKESGAKEVHIRVASPPIKYPCFMGINIPTKEELIANKPEFDHLAEYLGANSVVYLSVEGLVSSVQEGIKFKKQKEKKHDIMIQENGNGLECFEKSGHCTACLTGKYPVELEW

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
92
Zebrafish
91
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q06203 作为底物

Site PTM Type Enzyme
M1 Acetylation
T52 Phosphorylation
K62 Acetylation
K62 Methylation
K62 Ubiquitination
K65 Ubiquitination
K80 Ubiquitination
K81 Acetylation
K81 Ubiquitination
Y94 Phosphorylation
K99 Acetylation
K99 Ubiquitination
R130 Methylation
T166 Phosphorylation
K178 Ubiquitination
K220 Ubiquitination
C339 S-Nitrosylation
C348 S-Nitrosylation
K349 Ubiquitination
T356 Phosphorylation
K371 Acetylation
K372 Acetylation
K372 Ubiquitination
K381 Ubiquitination
K383 Methylation
S399 Phosphorylation
K403 Ubiquitination
K406 Ubiquitination
S408 Phosphorylation
K411 Ubiquitination
K482 Acetylation
K499 Acetylation
K510 Ubiquitination

研究背景

组织特异性:

Ubiquitously expressed.

亚基结构:

Homotetramer.

蛋白家族:

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

研究领域

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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