产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12127, RRID:AB_2844932.
展开/折叠
2 oxoglutarate dehydrogenase; 2 oxoglutarate dehydrogenase complex component E1; 2 oxoglutarate dehydrogenase mitochondrial; 2-oxoglutarate dehydrogenase; 2-oxoglutarate dehydrogenase complex component E1; AKGDH; Alpha ketoglutarate dehydrogenase; Alpha-ketoglutarate dehydrogenase; E1k; mitochondrial; ODO1_HUMAN; OGDC; OGDC E1; OGDC-E1; OGDH; Oxoglutarate (alpha ketoglutarate) dehydrogenase (lipoamide); Oxoglutarate decarboxylase; Oxoglutarate dehydrogenase (lipoamide); Oxoglutarate dehydrogenase (succinyl transferring);
抗原和靶标
- Q02218 ODO1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFHLRTCAAKLRPLTASQTVKTFSQNRPAAARTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLPLSRGSLAAVAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLDESDLDKVFHLPTTTFIGGQESALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDKSSENGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDVKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWRSTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDKICEEAFARSKDEKILHIKHWLDSPWPGFFTLDGQPRSMSCPSTGLTEDILTHIGNVASSVPVENFTIHGGLSRILKTRGEMVKNRTVDWALAEYMAFGSLLKEGIHIRLSGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q02218 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S24 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| K271 | Acetylation | Uniprot | |
| K276 | Acetylation | Uniprot | |
| Y304 | Phosphorylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| T367 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K401 | Acetylation | Uniprot | |
| K402 | Acetylation | Uniprot | |
| T462 | Phosphorylation | Uniprot | |
| K499 | Acetylation | Uniprot | |
| Y527 | Phosphorylation | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| K534 | Ubiquitination | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| Y555 | Phosphorylation | Uniprot | |
| K561 | Acetylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| K564 | Ubiquitination | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| K697 | Acetylation | Uniprot | |
| T858 | Phosphorylation | Uniprot | |
| K899 | Acetylation | Uniprot | |
| K899 | Ubiquitination | Uniprot | |
| Y909 | Phosphorylation | Uniprot | |
| T928 | Phosphorylation | Uniprot | |
| S934 | Phosphorylation | Uniprot | |
| K943 | Acetylation | Uniprot | |
| K947 | Ubiquitination | Uniprot | |
| Y966 | Phosphorylation | Uniprot | |
| Y968 | Phosphorylation | Uniprot | |
| K970 | Acetylation | Uniprot | |
| K970 | Ubiquitination | Uniprot | |
| K999 | Acetylation | Uniprot | |
| K999 | Ubiquitination | Uniprot | |
| K1000 | Ubiquitination | Uniprot | |
| T1012 | Phosphorylation | Uniprot |
研究背景
2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.
Mitochondrion matrix. Nucleus.
Note: Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation.
The 2-oxoglutarate dehydrogenase complex is composed of OGDH (2-oxoglutarate dehydrogenase; E1), DLST (dihydrolipoamide succinyltransferase; E2) and DLD (dihydrolipoamide dehydrogenase; E3). It contains multiple copies of the three enzymatic components (E1, E2 and E3). In the nucleus, the 2-oxoglutarate dehydrogenase complex associates with KAT2A.
Belongs to the alpha-ketoglutarate dehydrogenase family.
研究领域
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Amino acid metabolism > Lysine degradation.
· Metabolism > Amino acid metabolism > Tryptophan metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
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