LONP1 Antibody - #DF12119

Figure 3 NAD+ boosters reinforce UPRmt/mitophagy-related mitochondrial quality control in PD cells: a,b) Western blot data showing expression of mitophagy and UPRmt-related proteins in SH-SY5Y cells under different doses of NMN. c,d) Quantification of changes in the proteins shown in a, b (n = 3 independent samples; One-way ANOVA). β-actin was used as loading control for ATF4, ATF5 and LONP1. GAPDH was used as loading control for PINK1, OPTN and Parkin. e,f) Western blot data showing mitophagy and UPRmt-related protein expressions in SH-SY5Y cells under different elevated NAD+ pathways. g,h) Quantification of changes in the proteins shown in e, f (n = 4 independent samples; One-way ANOVA). β-actin was used as loading control for ATF4, ATF5 and LONP1. GAPDH was used as loading control for PINK1, OPTN and Parkin. i–k) Expression level of intracellular ATF4 under different treatments. For (i), scale bars, 15 µm (n = 3 independent samples; One-way ANOVA). l,m) Real-time PCR showing transcript levels of intracellular UPRmt and mitophagy indicators under different treatments (n = 3 independent samples; One-way ANOVA). Data are shown as mean ± SEM. The p values are indicated on the graphs. NS, not significant.