GLUD1 Antibody - #DF12116

(4)  
产品: GLUD1 抗体
货号: DF12116
描述: Rabbit polyclonal antibody to GLUD1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit, Chicken, Xenopus
分子量: 45-55 kDa; 61kD(Calculated).
蛋白号: P00367
RRID: AB_2844921

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Sheep(100%), Rabbit(100%), Chicken(91%), Xenopus(100%)
克隆:
Polyclonal
特异性:
GLUD1 Antibody detects endogenous levels of total GLUD1.
RRID:
AB_2844921
引用格式: Affinity Biosciences Cat# DF12116, RRID:AB_2844921.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AI118167; DHE3_HUMAN; EC 1.4.1.3; GDH 1; GDH; Gdh-X; GDH1; GLUD; Glud1; Glud1a; Glud1b; Gludl; Glutamate dehydrogenase (NAD(P)+); Glutamate dehydrogenase 1; Glutamate dehydrogenase 1 mitochondrial; Glutamate dehydrogenase 1a; Glutamate dehydrogenase 1b; Memory-related gene 2 protein; MGC127177; MGC132003; MGC80801; MGC93608; mitochondrial; MRG-2;

抗原和靶标

免疫原:
Uniprot:

P00367(DHE3_HUMAN) >>Visit HPA database.

基因/基因ID:
GLUD1(2746)
序列:
MYRYLGEALLLSRAGPAALGSASADSAALLGWARGQPAAAPQPGLALAARRHYSEAVADREDDPNFFKMVEGFFDRGASIVEDKLVEDLRTRESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTMELAKKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKPYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Xenopus
100
Rabbit
100
Chicken
91
Pig
0
Horse
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P00367 作为底物

Site PTM Type Enzyme
K68 Acetylation
R76 Methylation
S79 Phosphorylation
K84 Acetylation
K84 Ubiquitination
K98 Acetylation
C112 S-Nitrosylation
S128 Phosphorylation
Y135 Phosphorylation
S153 Phosphorylation
T154 Phosphorylation
S157 Phosphorylation
K162 Acetylation
K162 Ubiquitination
Y170 Phosphorylation
K171 Methylation
K183 Sumoylation
K183 Ubiquitination
K187 Ubiquitination
K191 Ubiquitination
T194 Phosphorylation
K200 Ubiquitination
T206 Phosphorylation
K211 Acetylation
K211 Ubiquitination
K212 Ubiquitination
S227 Phosphorylation
T228 Phosphorylation
K302 Methylation
S316 Phosphorylation
S333 Phosphorylation
S336 Phosphorylation
K346 Acetylation
K352 Acetylation
K352 Ubiquitination
S357 Phosphorylation
K363 Ubiquitination
K365 Ubiquitination
Y367 Phosphorylation
S370 Phosphorylation
S384 Phosphorylation
K386 Ubiquitination
K390 Acetylation
K390 Ubiquitination
K399 Ubiquitination
T409 Phosphorylation
T410 Phosphorylation
K415 Acetylation
K415 Ubiquitination
S438 Phosphorylation
Y439 Phosphorylation
S450 Phosphorylation
Y451 Phosphorylation
K457 Acetylation
S462 Phosphorylation
Y464 Phosphorylation
K480 Acetylation
K480 Ubiquitination
R496 Methylation
K503 Acetylation
K503 Ubiquitination
Y512 Phosphorylation
T513 Phosphorylation
K527 Acetylation
K527 Ubiquitination
Y528 Phosphorylation
Y539 Phosphorylation
K545 Acetylation
K545 Ubiquitination
Y550 Phosphorylation

研究背景

功能:

Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).

翻译修饰:

ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity (By similarity). Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homohexamer.

蛋白家族:

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Metabolism of other amino acids > D-Glutamine and D-glutamate metabolism.

· Metabolism > Energy metabolism > Nitrogen metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

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