CCT3 Antibody - #DF12113

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产品: CCT3 抗体
货号: DF12113
描述: Rabbit polyclonal antibody to CCT3
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 60 kDa; 61kD(Calculated).
蛋白号: P49368
RRID: AB_2844918

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
预测:
Zebrafish(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
CCT3 Antibody detects endogenous levels of total CCT3.
RRID:
AB_2844918
引用格式: Affinity Biosciences Cat# DF12113, RRID:AB_2844918.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CCT 3; CCT gamma; CCT-gamma; CCT3; CCTG; Chaperonin containing TCP1 subunit 3 (gamma); Chaperonin containing TCP1 subunit 3; hTRiC5; PIG48; T complex protein 1 gamma subunit; T complex protein 1 subunit gamma; T-complex protein 1 subunit gamma; TCP 1 gamma; TCP-1-gamma; TCP1 (t complex 1) ring complex polypeptide 5; TCPG_HUMAN; TRIC5;

抗原和靶标

免疫原:
Uniprot:

P49368(TCPG_HUMAN) >>Visit HPA database.

基因/基因ID:
CCT3(7203)
序列:
MMGHRPVLVLSQNTKRESGRKVQSGNINAAKTIADIIRTCLGPKSMMKMLLDPMGGIVMTNDGNAILREIQVQHPAAKSMIEISRTQDEEVGDGTTSVIILAGEMLSVAEHFLEQQMHPTVVISAYRKALDDMISTLKKISIPVDISDSDMMLNIINSSITTKAISRWSSLACNIALDAVKMVQFEENGRKEIDIKKYARVEKIPGGIIEDSCVLRGVMINKDVTHPRMRRYIKNPRIVLLDSSLEYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Interacts with DNAAF4 (By similarity).

蛋白家族:

Belongs to the TCP-1 chaperonin family.

文献引用

1). Integrated analyzes identify CCT3 as a modulator to shape immunosuppressive tumor microenvironment in lung adenocarcinoma. BMC CANCER, 2023 (PubMed: 36918801) [IF=3.8]

Application: WB    Species: Human    Sample: LUAD A549 cells

Fig. 11 CCT3 knockdown suppressed colony formation and cell proliferation but induced apoptosis of LUAD A549 cells. (a, b) Western blotting (a) and band intensity (b) analyzes of small interfering RNA mediated CCT3 knockdown in A549 cells, GAPDH was used as the loading control. (c, d) Representative colony formation (c) and comparisons of colony counts (d) between si-CCT3 and si-Control cells. (e) The proliferation of si-CCT3 and si-Control A549 cells were determined by WST-8/Cell Counting Kit-8 at 0, 24 and 48 h after cell culturing. (f, g) Representative flow cytometry (f) and comparisons of percentages of total Annexin-V+ cells (g) between si-CCT3 and si-Control A549 cells. Data are representative of three independent experiments. **P 

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