Spartin, SPG20 Antibody - #DF12086

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产品: Spartin, SPG20 抗体
货号: DF12086
描述: Rabbit polyclonal antibody to Spartin, SPG20
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 75 kDa,84 kDa; 73kD(Calculated).
蛋白号: Q8N0X7
RRID: AB_2844891

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Bovine(100%), Horse(92%), Sheep(92%), Rabbit(92%), Chicken(83%), Xenopus(83%)
克隆:
Polyclonal
特异性:
Spartin, SPG20 Antibody detects endogenous levels of total Spartin, SPG20.
RRID:
AB_2844891
引用格式: Affinity Biosciences Cat# DF12086, RRID:AB_2844891.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

SPARTIN; Spastic paraplegia 20 (Troyer syndrome); TAHCCP1; Trans activated by hepatitis C virus core protein 1;

抗原和靶标

免疫原:
Uniprot:

Q8N0X7(SPART_HUMAN) >>Visit HPA database.

基因/基因ID:
SPART(23111)
表达:
Q8N0X7 SPART_HUMAN:

Ubiquitously expressed, with highest levels of expression detected in adipose tissue.

序列:
MEQEPQNGEPAEIKIIREAYKKAFLFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSKESEHTGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLQNDLQEVPKLYPEFPPKDMCEKLPEPQSFSSAPQHAEVNGNTSTPSAGAVAAPASLSLPSQSCPAEAPPAYTPQAAEGHYTVSYGTDSGEFSSVGEEFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTAGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEEENEFQIPGRTRPSSDQLKEASGTDVKQLDQGNKDVRHKGKRGKRAKDTSSEEVNLSHIVPCEPVPEEKPKELPEWSEKVAHNILSGASWVSWGLVKGAEITGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDKDGKSPLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGYNAGEATHHAVDSAVNVGVTAYNINNIGIKAMVKKTATQTGHTLLEDYQIVDNSQRENQEGAANVNVRGEKDEQTKEVKEAKKKDK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Pig
92
Horse
92
Sheep
92
Rabbit
92
Xenopus
83
Chicken
83
Dog
79
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8N0X7 作为底物

Site PTM Type Enzyme
M1 Acetylation
K29 Ubiquitination
K39 Ubiquitination
Y45 Phosphorylation
Y46 Phosphorylation
K47 Ubiquitination
S58 Phosphorylation
K62 Ubiquitination
K82 Ubiquitination
K97 Ubiquitination
T101 Phosphorylation
S102 Phosphorylation
K112 Ubiquitination
Y114 Phosphorylation
K287 Ubiquitination
R334 Methylation
S357 Phosphorylation
S358 Phosphorylation
K362 Methylation
K362 Ubiquitination
K370 Methylation
K370 Ubiquitination
K377 Ubiquitination
K382 Methylation
K390 Ubiquitination
S393 Phosphorylation
S400 Phosphorylation
K412 Ubiquitination
K414 Ubiquitination
K422 Ubiquitination
K440 Ubiquitination
K447 Ubiquitination
K451 Ubiquitination
K455 Ubiquitination
K465 Ubiquitination
S470 Phosphorylation
K475 Ubiquitination
K481 Ubiquitination
K489 Acetylation
K489 Ubiquitination
C499 S-Nitrosylation
C504 S-Nitrosylation
K507 Ubiquitination
K514 Ubiquitination
K519 Ubiquitination
S524 Phosphorylation
K526 Ubiquitination
K527 Ubiquitination
K529 Ubiquitination
K532 Ubiquitination
K578 Sumoylation
K578 Ubiquitination
K615 Ubiquitination
Y628 Phosphorylation
K651 Ubiquitination

研究背景

功能:

May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis.

翻译修饰:

Ubiquitinated; ubiquitination does not require ITCH and WWP1.

细胞定位:

Cytoplasm. Midbody.
Note: Transiently associated with endosomes (PubMed:19580544). Colocalized with IST1 to the ends of Flemming bodies during cytokinesis (PubMed:20719964).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed, with highest levels of expression detected in adipose tissue.

亚基结构:

Interacts with ITCH and WWP1. Interacts (via MIT domain) with IST1; leading to the recruitment of SPART to midbodies.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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