SKAP2 Antibody - #DF12085

Fig. 4 Aberrant RNA and protein profiles in KI mice. a Volcano plot of differentially expressed genes (DEGs) identified by single-cell RNA-seq in round spermatids from wild-type (WT) and knock-in (KI) mice at postnatal day 56 (P56). Downregulated genes are shown in blue, upregulated genes in red. b Gene Ontology (GO) enrichment analysis of DEGs highlights significant involvement in biological processes related to cytoskeletal organization and sperm motility. c Violin plots of Skap2 mRNA expression in WT and KI round spermatids. Statistical significance was assessed using a Mann–Whitney U-test. d RT-qPCR validation of Skap2, a gene implicated in sperm cytoskeletal dynamics, confirms significantly reduced expression in purified round spermatids. Data are presented as mean ± SD; significance determined by a two-tailed Student’s t-test. n = 6 per group. e Venn diagram showing overlapping differentially expressed proteins identified in human sperm, mouse sperm, and mouse testis. f GO enrichment analysis of the overlapping proteins from (e) reveals significant enrichment in biological processes associated with cytoskeletal organization and F-ACTIN assembly. g Venn diagram illustrating the intersection between DEGs and differentially expressed proteins. h Doughnut chart showing that a majority (71.08%) of dysregulated proteins are associated with the sperm cytoskeleton, with a substantial fraction (77.15%) specifically linked to sperm acrosome-related structures. i, j Heatmaps depicting expression patterns of key proteins involved in cytoskeletal organization (i) and F-ACTIN assembly (j); color intensity represents relative expression levels. k Representative western blots showing decreased protein expression in KI round spermatids compared with WT; GAPDH served as a loading control. l Quantification of protein levels from (k) confirms significant reductions in KI versus WT. Data are presented as mean ± SD; statistical significance was determined by a two-sided Student’s t-test. n = 6 per group