Kindlin 2 Antibody - #DF12078

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产品: Kindlin 2 抗体
货号: DF12078
描述: Rabbit polyclonal antibody to Kindlin 2
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 78 kDa; 78kD(Calculated).
蛋白号: Q96AC1
RRID: AB_2844883

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(92%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
Kindlin 2 Antibody detects endogenous levels of total Kindlin 2.
RRID:
AB_2844883
引用格式: Affinity Biosciences Cat# DF12078, RRID:AB_2844883.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ34213; FLJ44462; UNC112; FERM2_HUMAN; Fermitin family homolog 2; Fermt2; KIND2; Kindlin-2; MIG-2; MIG2; Mitogen-inducible gene 2 protein; PH domain-containing family C member 1; Pleckstrin homology domain containing family C (with FERM domain) member 1; Pleckstrin homology domain-containing family C member 1; PLEKHC1; UNC112B;

抗原和靶标

免疫原:
Uniprot:

Q96AC1(FERM2_HUMAN) >>Visit HPA database.

基因/基因ID:
FERMT2(10979)
表达:
Q96AC1 FERM2_HUMAN:

Ubiquitous. Found in numerous tumor tissues.

序列:
MALDGIRMPDGCYADGTWELSVHVTDLNRDVTLRVTGEVHIGGVMLKLVEKLDVKKDWSDHALWWEKKRTWLLKTHWTLDKYGIQADAKLQFTPQHKLLRLQLPNMKYVKVKVNFSDRVFKAVSDICKTFNIRHPEELSLLKKPRDPTKKKKKKLDDQSEDEALELEGPLITPGSGSIYSSPGLYSKTMTPTYDAHDGSPLSPTSAWFGDSALSEGNPGILAVSQPITSPEILAKMFKPQALLDKAKINQGWLDSSRSLMEQDVKENEALLLRFKYYSFFDLNPKYDAIRINQLYEQAKWAILLEEIECTEEEMMMFAALQYHINKLSIMTSENHLNNSDKEVDEVDAALSDLEITLEGGKTSTILGDITSIPELADYIKVFKPKKLTLKGYKQYWCTFKDTSISCYKSKEESSGTPAHQMNLRGCEVTPDVNISGQKFNIKLLIPVAEGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNLEVQNILSFLKMQHLNPDPQLIPEQITTDITPECLVSPRYLKKYKNKQITARILEAHQNVAQMSLIEAKMRFIQAWQSLPEFGITHFIARFQGGKKEELIGIAYNRLIRMDASTGDAIKTWRFSNMKQWNVNWEIKMVTVEFADEVRLSFICTEVDCKVVHEFIGGYIFLSTRAKDQNESLDEEMFYKLTSGWV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.

细胞定位:

Cytoplasm. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Stress fiber. Cell junction>Focal adhesion. Membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Peripheral membrane protein>Cytoplasmic side. Nucleus. Cytoplasm>Myofibril>Sarcomere>I band. Cell surface.
Note: Colocalizes with actin stress fibers at cell-ECM focal adhesion sites. Colocalizes with ITGB3 at lamellipodia at the leading edge of spreading cells. Binds to membranes that contain phosphatidylinositides.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Found in numerous tumor tissues.

亚基结构:

Interacts with ILK (By similarity). Interacts with FBLIM1. Interacts with ITGB1 and ITGB3. Interacts with active, unphosphorylated CTNNB1. Identified in a complex with CTNNB1 and TCF7L2/TCF4. Interacts with ITGB1; the interaction is inhibited in presence of ITGB1BP1.

蛋白家族:

The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.

The PH domain binds phospholipids. Binds preferentially phosphatidylinositol-3,4,5-trisphosphate, and has lower affinity for phosphatidylinositol-4,5-bisphosphate (PubMed:22030399).

The N-terminal region displays a ubiquitin-type fold and mediates interaction with membranes containing negatively charged phosphatidylinositol phosphate via a surface enriched in positively charged residues.

Belongs to the kindlin family.

文献引用

1). YBX1 knockdown induces renal cell carcinoma cell apoptosis via Kindlin-2. Cell Cycle, 2021 (PubMed: 34709966) [IF=3.4]

Application: WB    Species: Human    Sample: renal cancer cells

Figure 1. YBX1 regulates the expression of Kindlin-2 in renal cancer cells. (a) GSEA analyzes genes positively or negatively regulated by YBX1, and the enrichment map shows that the differentially expressed genes are significantly related to EMT. (b) The heat map shows differently expressed genes after YBX1 knockdown during the EMT process. (c) The mRNA levels of Kindlin-2 were examined by qRT-PCR in YBX1 knockdown ACHN (left panel) and 786-O (right-panel) cells. (d) Kindlin-2 protein expressions in YBX1 knockdown ACHN (left panel) and 786-O (right panel) cells were detected by western blot assay. Scr: down-expression empty plasmid control; shYBX1: YBX1 knockdown; Statistically significant differences were indicated

Application: IHC    Species: Human    Sample: renal cancer cells

Figure 2. YBX1 and Kindlin-2 are elevated in RCC and high YBX1 expression correlates with poor prognosis in renal cancer. (a) Relative expression of YBX1 and Kindlin-2 proteins in 20 pairs of RCC tissues (T) and adjacent normal tissues (N) using western blot assay. Upper panel: representative four pairs of western blot images. Lower panel: the expressions of YBX1 and Kindlin-2 was quantified by normalizing with β-actin. The analysis was used paired t-test, P = 0.0059, P = 0.0334, respectively, n = 20. Representative images of immunohistochemical staining for YBX1 and Kindlin-2 are shown in (b). Scale bar = 50 μm. (c) High YBX1 expression correlates with poor prognosis in RCC patients. (http://www.oncolnc.org/, https://kmplot.com/analysis/) Statistically significant differences were indicated:

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