Kindlin 2 Antibody - #DF12078

Figure 1. YBX1 regulates the expression of Kindlin-2 in renal cancer cells. (a) GSEA analyzes genes positively or negatively regulated by YBX1, and the enrichment map shows that the differentially expressed genes are significantly related to EMT. (b) The heat map shows differently expressed genes after YBX1 knockdown during the EMT process. (c) The mRNA levels of Kindlin-2 were examined by qRT-PCR in YBX1 knockdown ACHN (left panel) and 786-O (right-panel) cells. (d) Kindlin-2 protein expressions in YBX1 knockdown ACHN (left panel) and 786-O (right panel) cells were detected by western blot assay. Scr: down-expression empty plasmid control; shYBX1: YBX1 knockdown; Statistically significant differences were indicated

Figure 2. YBX1 and Kindlin-2 are elevated in RCC and high YBX1 expression correlates with poor prognosis in renal cancer. (a) Relative expression of YBX1 and Kindlin-2 proteins in 20 pairs of RCC tissues (T) and adjacent normal tissues (N) using western blot assay. Upper panel: representative four pairs of western blot images. Lower panel: the expressions of YBX1 and Kindlin-2 was quantified by normalizing with β-actin. The analysis was used paired t-test, P = 0.0059, P = 0.0334, respectively, n = 20. Representative images of immunohistochemical staining for YBX1 and Kindlin-2 are shown in (b). Scale bar = 50 μm. (c) High YBX1 expression correlates with poor prognosis in RCC patients. (http://www.oncolnc.org/, https://kmplot.com/analysis/) Statistically significant differences were indicated: