HEY1 Antibody - #DF12076

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产品: HEY1 抗体
货号: DF12076
描述: Rabbit polyclonal antibody to HEY1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: Q9Y5J3
RRID: AB_2844881

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
HEY1 Antibody detects endogenous levels of total HEY1.
RRID:
AB_2844881
引用格式: Affinity Biosciences Cat# DF12076, RRID:AB_2844881.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Basic helix loop helix protein OAF1; bHLHb31; Cardiovascular helix loop-helix factor 2; Cardiovascular helix-loop-helix factor 2; CHF-2; CHF2; Class B basic helix-loop-helix protein 31; Hairy and enhancer of split related protein 1; Hairy and enhancer of split-related protein 1; Hairy enhancer-of-split related with YRPW motif protein 1; Hairy related transcription factor 1; Hairy-related transcription factor 1; Hairy/enhancer of split related with YRPW motif 1; Hairy/enhancer-of-split related with YRPW motif protein 1; HERP2; HES related repressor protein 1; HES-related repressor protein 1; HES-related repressor protein 2; HESR-1; HESR1; HEY 1; hey1; HEY1_HUMAN; hHRT1; HRT-1; HRT1; OAF1;

抗原和靶标

免疫原:

A synthesized peptide derived from human HEY1, corresponding to a region within the internal amino acids.

基因/基因ID:
HEY1(23462)

研究领域

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

文献引用

1). High-resolution subtyping of fibroblasts in gastric cancer reveals diversity among fibroblast subsets and an association between the MFAP5-fibroblast subset and immunotherapy. Frontiers in immunology, 2024 (PubMed: 39524442) [IF=5.7]

Application: WB    Species: human    Sample: HGC-27 cells

Figure 9. MFAP5 promotes the GC cell proliferation and migration. (A) Assessment of cell proliferation in hr MFAP5-treated HGC-27 cells. (B) Evaluation of cell migration in hr MFAP5-treated HGC-27 cells. (C) Western blotting to examine Notch2 and HEY1 levels in hr MFAP5-treated HGC-27 cells. (D) Kaplan-Meier analysis comparing high and low expression levels of NOTCH2 or HEY1 among patients in the TCGA-STAD cohort. hr MFAP5, human recombinant MFAP5. *p
2). γ‐secretase inhibitors suppress IL‐20‐mediated osteoclastogenesis via Notch signaling and are affected by Notch2 in vitro. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2022 (PubMed: 35384009) [IF=4.1]

Application: WB    Species: Human    Sample:

FIGURE 2 20 ng/mL IL-20 treated the pre-osteoclasts for 48 h meanwhile control groups were treated with 0 ng/mL IL-20. Detected mRNA and analysed by GO Analysis and Pathway Analysis. (A) Bubble chart analysis based on KEGG databases. (B) Gene Set Enrichment Analysis of osteoclast differentiation with control groups and IL-20 treat groups. (C) Gene heatmap showed four classical gene were enriched to Notch signal pathway (Red – up regulated and green – down regulated). (D-E) The expression of Notch1 and Notch2 mRNA with different concentration of IL-20 stimulation. Analysed by one-way ANOVA with Tukey's post hoc analysis with three independent experiments. *P < .05 vs the control group; ns, not significant. (F) mRNA expression of Notch1, Notch2, Hes1 and Hey1 at 20 ng/mL IL-20 stimulation using by qRT-PCR and analysed by Student's t test. Bars represent mean ± SEM from three independent experiments. *P < .05 vs the control group; ns, not significant. (G-H) Western blotting data and analysis of Notch signal pathway. Student's t test was used to detect difference. Bars represent mean ± SEM from three independent experiments. *P < .05 vs the control group; ns, not significant
3). Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats. Stem Cells International, 2019 (PubMed: 31249601) [IF=3.8]

Application: WB    Species: rat    Sample: MSCs

Figure 3|: Expression of NPC markers and Notch pathway in NPC exosome-treated MSCs. (a) Gene expression of NPC markers (collagen II,Aggrecan, and Sox9) was significantly upregulated in exosome-treated MSCs in 7, 14, and 21 days. (b) Expression of related protein was normalized to GAPDH. (c) Expression of Notch pathway-related genes in exosome-treated MSCs was detected by qRT-PCR. (d)Western blot analysis of Notch pathway-related protein in exosome-treated MSCs. All data were showed as mean ± SD. N = 3. ∗P < 0 05, ∗∗P < 0 01, and ∗∗∗P < 0 001.

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