eIF4A3 Antibody - #DF12071

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产品: eIF4A3 抗体
货号: DF12071
描述: Rabbit polyclonal antibody to eIF4A3
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
蛋白号: P38919
RRID: AB_2844876

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
eIF4A3 Antibody detects endogenous levels of total eIF4A3.
RRID:
AB_2844876
引用格式: Affinity Biosciences Cat# DF12071, RRID:AB_2844876.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD box protein 48; eIF-4A-III; eIF4A-III; EIF4A3; eIF4AIII; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; hNMP 265; IF4A3_HUMAN; NMP 265; NMP265; Nuclear matrix protein 265; NUK34;

抗原和靶标

免疫原:

A synthesized peptide derived from human eIF4A3, corresponding to a region within the internal amino acids.

基因/基因ID:
EIF4A3(9775)

研究领域

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Transcription > Spliceosome.

文献引用

1). The Combined Effects of circRNA Methylation Promote Pulmonary Fibrosis. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2022 (PubMed: 35213290) [IF=5.9]

Application: WB    Species: Human    Sample: lung fibroblasts

Figure 4. As the target proteins of hsa_circ_0000672 and hsa_circ_0005654, eIF4A3 promotes the activation, migration and activity of human lung fibroblasts. PCR was used to detect the expression of hsa_circ_0000672 and hsa_circ_0005654 in human lung fibroblasts at different time points. The data are presented as the mean ± SD, n = 3. (B) In situ hybridization showed that hsa_circ_0000672 and hsa_circ_0005654 were present in the cytoplasm. Scale bars, 20 μm. (C) Bioinformatics analysis showed that hsa_circ_0000672 and hsa_circ_0005654 pre-mRNAs have a common RNA binding protein (RBP). (D) The RIP test was used to detect the interaction of hsa_circ_0000672 and hsa_circ_0005654 with eIF4A3. (E and F) Knockdown efficiency of EIF4A3. The data are presented as the mean ± SD, n = 3. *P 
2). circ_SIRT1 upregulates ATG12 to facilitate Imatinib resistance in CML through interacting with EIF4A3. Gene, 2024 (PubMed: 37866664) [IF=2.6]

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