RBM15 Antibody - #DF12061

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产品: RBM15 抗体
货号: DF12061
描述: Rabbit polyclonal antibody to RBM15
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q96T37
RRID: AB_2844866

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
RBM15 Antibody detects endogenous levels of total RBM15.
RRID:
AB_2844866
引用格式: Affinity Biosciences Cat# DF12061, RRID:AB_2844866.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

One twenty two protein; One-twenty two protein 1; OTT; OTT1; Putative RNA-binding protein 15; RBM15; RBM15_HUMAN; RNA binding motif protein 15; RNA-binding motif protein 15;

抗原和靶标

免疫原:

A synthesized peptide derived from human RBM15, corresponding to a region within N-terminal amino acids.

基因/基因ID:
RBM15(64783)

文献引用

1). Effect of the m6ARNA gene on the prognosis of thyroid cancer, immune infiltration, and promising immunotherapy. Frontiers in Immunology, 2022 (PubMed: 36389678) [IF=5.7]

Application: IHC    Species: Human    Sample:

Figure 10 Representative immunohistochemical staining of RBM15, KIAA1429, FTO in benign thyroid nodule tissue and thyroid cancer tissue. (A) Immunohistochemical image of FTO in thyroid cancer tissue. (B) Immunohistochemical image of FTO in benign thyroid nodule tissue.(C) Immunohistochemical image of RBM15 in PTC tissue. (D) Immunohistochemical staining image of RBM15 in benign thyroid nodule tissue. (E) Immunohistochemical image of KIAA1429 in PTC tissue. (F) Immunohistochemical image of KIAA1429 in benign thyroid nodule tissue.
2). RBM15-mediated m6A modification of XPR1 promotes the malignant progression of lung adenocarcinoma. Naunyn-Schmiedeberg's archives of pharmacology, 2025 (PubMed: 39928150) [IF=3.1]

Application: WB    Species: human    Sample: LUAD cells

Fig. 3 RBM15 stabilized XPR1 mRNA expression through m6A modification in LUAD cells. (A and B) The dual-luciferase reporter assay and MeRIP assay were performed to identify the association between RBM15 and XPR1. (C) The actinomycin D assay was performed to analyze the effect of RBM15 silencing on the stability of XPR1 mRNA in both A549 and PC-9 cells. (D) The effects of RBM15 silencing on RBM15 and XPR1 protein expression were analyzed by western blotting assay. (E) RBM15 expression was analyzed through the TCGA database in LUAD tissues and normal lung tissues. (F) The IHC assay was used to analyze RBM15 protein expression in clinical LUAD tissues and normal lung tissues. (G) RBM15 mRNA expression was quantified by qRT-PCR in clinical LUAD tissues and normal lung tissues. (H and I) The western blotting assay was used to analyze RBM15 protein expression in clinical LUAD tissues, normal lung tissues, 16HBE cells, A549 cells and PC-9 cells. (J and K) The correlation analysis of RBM15 and XPR1 in LUAD tissues. **P 

Application: IHC    Species: Mouse    Sample:

Fig. 5 RBM15 silencing delayed tumor growth by regulating XPR1 in vivo. A549 cells were injected subcutaneously into the flanks of the mice following intratumor injection with sh-RBM15, XPR1, sh-NC and/or vector. Tumor volume was analyzed every 1 week (A), and the tumors were harvested after 4 weeks for tumor weight analysis (B) and protein expression analysis of RBM15, XPR1 and ki-67 (C). **P 
3). SILENCING M 6 A READER YTHDC1 REDUCES INFLAMMATORY RESPONSE IN SEPSIS-INDUCED CARDIOMYOPATHY BY INHIBITING SERPINA3N EXPRESSION. Shock (Augusta, Ga.), 2023 (PubMed: 36877222) [IF=2.7]

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