产品: Transgelin 2 抗体
货号: DF12053
描述: Rabbit polyclonal antibody to Transgelin 2
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 22 kDa; 22kD(Calculated).
蛋白号: P37802
RRID: AB_2844858

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
Transgelin 2 Antibody detects endogenous levels of total Transgelin 2.
RRID:
AB_2844858
引用格式: Affinity Biosciences Cat# DF12053, RRID:AB_2844858.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDABP0035; epididymis tissue protein Li 7e; HA1756; KIAA0120; SM22-alpha homolog; TAGL2_HUMAN; Tagln2; Transgelin-2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P37802 TAGL2_HUMAN:

Expressed in epididymis (at protein level).

序列:
MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALYPEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
82
Xenopus
75
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

组织特异性:

Expressed in epididymis (at protein level).

蛋白家族:

Belongs to the calponin family.

文献引用

1). TAGLN2 induces resistance signature ISGs by activating AKT-YBX1 signal with dual pathways and mediates the IFN-related DNA damage resistance in gastric cancer. Cell death & disease, 2024 (PubMed: 39168971) [IF=8.1]

Application: IHC    Species: Human    Sample: gastric cancer (GC) tissue

Fig. 1. Aberrantly upregulated expression of TAGLN2 results in the induction of ISGs. A Aberrantly upregulated TAGLN2 protein expression was assessed by IHC analysis in gastric cancer (GC) tissue microarray (TMA) chips containing 75 tumor tissues and their paired normal counterparts. B Cellular TAGLN2 expression in tumor tissues vs. paired normal counterparts was evaluated in the membrane, cytoplasm and nucleus. Distant metastases (M), T stage and overall cancer stage (Stage I~IV) were significantly correlated with TAGLN2 expression in the cytoplasm. C Representative IHC images show that abnormal TAGLN2 expression level increases as GC progresses (Stage I~IV). The results were obtained by multiplying the PP by the IS (immunoreactive score = PP × IS). *P 

2). Comparative proteomics identify HSP90A, STIP1 and TAGLN‑2 in serum extracellular vesicles as potential circulating biomarkers for human adenomyosis. Experimental and Therapeutic Medicine, 2022 (PubMed: 35495589) [IF=2.4]

Application: WB    Species: human    Sample: T‑AMEVs and B‑AMEVs

Figure 4. | HSP90A, STIP1 and TAGLN‑2 are expressed in B‑AMEVs and T‑AMEVs. (D and E) HSP90A, STIP1 and TAGLN‑2 were present in T‑AMEVs and B‑AMEVs from patients with adenomyosis,but not in those of patients in the control group.

3). Overexpression of TAGLN2 regulated by non-coding RNAs refering to poor prognosis and tumor immune cell infiltration in hepatocellular carcinoma. Research Square, 2023

Application: WB    Species: Human    Sample: HCC tissues

Figure 10. Validation results of TAGLN2 expression in HCC. (a) qRT-PCR analysis of TAGLN2 mRNA expression in 20 pairs of HCC tissues and adjacent non-tumor tissues. (b) Western blot detection of TAGLN2 protein expression and relative quantification in the mean values of three HCC tumor tissues and three neighboring tissues showed elevated TAGLN2 protein expression in HCC tumor tissues. (c) IHC analysis of TAGLN2 stained significantly darker in tumor tissues and had higher protein expression levels than adjacent tissues. (*P < 0.05, **P< 0.01, ***P < 0.001).

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.