NBR1 Antibody - #DF12049

Figure 4 ｜ Effect of bradykinin postconditioning on the immunopositivity of AMPK/ mTOR signaling pathway, autophagy-related proteins, and brain injury marker in rats with restoration of spontaneous circulation, as detected by immunohistochemistry.

Figure 4 ｜ Effect of bradykinin postconditioning on the immunopositivity of AMPK/ mTOR signaling pathway, autophagy-related proteins, and brain injury marker in rats with restoration of spontaneous circulation, as detected by immunohistochemistry. Compared with the Sham group, p-mTOR and p62 immunopositivity in the ROSC group was significantly decreased, S100β, p-AMPK, and LC3 immunopositivity was significantly increased, and NBR1 immunopositivity remained unchanged. Compared with the ROSC group, p-mTOR, p62, and S100β immunopositivity in the BK group was significantly decreased, while p-AMPK, NBR1, and LC3 immunopositivity was significantly increased. Compared with the BK group, LC3, p-AMPK, and NBR1 immunopositivity in the CP+BK group was significantly decreased, whereas S100β, p62, and p-mTOR immunopositivity was significantly increased; LC3, p-AMPK, and NBR1 immunopositivity in the Ra+BK group was significantly increased, while S100β, p62, and p-mTOR immunopositivity was significantly decreased. Scale bars: 50 μm. All data are presented as the mean ± SD (n = 8). *P < 0.05, **P < 0.01, vs. Sham group; #P < 0.05, ##P < 0.01, vs. ROSC group; †P < 0.05, ††P < 0.01, vs. BK group (one-way analysis of variance followed by Tukey’s post hoc test). BK: Bradykinin; CP: compound C; IOD: integrated optical density; LC3: microtubule-associated protein 1 light chain 3; NBR1: neighbor of breast cancer 1 gene; p-AMPK: phosphorylated adenosine-monophosphate activated protein kinase; p-mTOR: p-mechanistic target of rapamycin; Ra: rapamycin; ROSC: restoration of spontaneous circulation; S100β: S100 calcium-binding protein B; Sham: sham operation.