PFKFB3 Antibody - #DF12016

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产品: PFKFB3 抗体
货号: DF12016
描述: Rabbit polyclonal antibody to PFKFB3
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
蛋白号: Q16875
RRID: AB_2844821

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PFKFB3 Antibody detects endogenous levels of total PFKFB3.
RRID:
AB_2844821
引用格式: Affinity Biosciences Cat# DF12016, RRID:AB_2844821.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2;

抗原和靶标

免疫原:

A synthesized peptide derived from human PFKFB3, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PFKFB3(5209)

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

文献引用

1). Rosmarinic Acid Alleviates Radiation-Induced Pulmonary Fibrosis by Downregulating the tRNA N7-Methylguanosine Modification-Regulated Fibroblast-to-Myofibroblast Transition Through the Exosome Pathway. Journal of inflammation research, 2024 (PubMed: 39188632) [IF=4.5]

Application: WB    Species: Mouse    Sample: MLFs

Figure 7 RA diminished glycolysis by reducing acetylated PFKFB3 and cytoplasmic translocation. (A) ECARs were evaluated via a Seahorse XFe96 Extracellular Flux Analyzer. (B) The acetylation levels of PFKFB3 were examined via Western blotting. (C) After treatment with Remodelin (an inhibitor of NAT10), the acetylation levels of PFKFB3 in MLFs were examined. (D) The acetylation sites of PFKFB3 were examined by immunoprecipitation. (E) The effects of the K472Q and K473Q mutations on the distribution of PFKFB3 in MLFs were assessed via immunofluorescence staining. Scale bars, 50 μm. (F) Phosphorylation levels of PFKFB3 and AMPK were examined. (G and H) The effects of the K472Q and K473Q mutations on the phosphorylation levels of PFKFB3 were examined. (I) The effects of the K472Q and K473Q mutations on the ECAR were evaluated via the Seahorse assay.
2). Yes associated protein 1 promotes resistance to 5-fluorouracil in gastric cancer by regulating GLUT3-dependent glycometabolism reprogramming of tumor-associated macrophages. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2021 (PubMed: 33727040) [IF=3.8]

Application: WB    Species: Human    Sample: Gastric cancer (GC) cells

Fig. 3. IL13 secreted by YAP1-overexpressed GC cells stimulates resistance to 5-FU via inducing M2 subtype macrophage glycolysis reprogramming. A-C. RT-PCR was used to detect the mRNA expression of glycolysis enzymes, fatty acid and amino acid metabolism enzymes in THP1 after co-cultured with MKN-YAP1 or MKN45-Vetor. D. Protein level change of glycolysis enzymes in THP1 after co-cultured with MKN-YAP1, MKN45-Vetor, SGC7901-siYAP1 or SGC7901-NC. E. RT-PCR revealed the mRNA expression of glycolysis enzymes and M2 TAMs markers in THP1 after co-cultured with SGC7901-siYAP1 or SGC7901-NC. F-G. Relative lactate release from cells was determined by colorimetric analysis. Relative glucose uptake cells by Flow cytometry detection. All data presented are the mean ± SD (*p < 0.05, **p < 0.01) of triplicate determination from three independent experiments.
3). PFKFB3 facilitates cell proliferation and migration in anaplastic thyroid carcinoma via the WNT/β-catenin signaling pathway. Endocrine, 2024 (PubMed: 38378893) [IF=3.0]
4). Effect of exercise training on cardiac function and glucose metabolism in the ischemic border zone: insights from multi-modal imaging techniques. Frontiers in cardiovascular medicine, 2025 (PubMed: 40520940) [IF=2.8]

Application: IHC    Species: Rat    Sample: myocardial tissues

Figure 6. Effects of exercise on the expressions of GLUT4 and PFKFB3 in myocardial tissues of rats after 8 weeks of exercise training. (A,C) Representative micrographs of heart sections from the ischemic border zones of the three groups. (B,D) Protein expressional levels of GLUT4 and PFKFB3 in the Sham, MIC and MIE groups. n = 4 rats per group. ***P 
5). STAT3 signaling mediates peritoneal fibrosis by activating hyperglycolysis. American journal of translational research, 2022 (PubMed: 36398234) [IF=1.7]

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