MFF Antibody - #DF12006

FIGURE 8 | PGC-1α might be important in mediating the effects of PF on TNF-α-treated C2C12 cells.(I) Representative western blots using antibodies against p-DRP1 (Ser616), DRP1, FIS1, MFF, MTFP1 and GAPDH.

Figure 4 miR-27b reduces mitochondrial fission by targeting Mff. (A, B), RT-qPCR detection of miR-27b and Mff mRNA expression levels in each cell group. (C–F), Quantitative analysis of Mff, Drp1, Mid49, and Mid51 protein expression levels by Western blot. (G), Representative Western blot bands for each group. (H), Dual-luciferase reporter assay verifying the targeting relationship between miR-27b and Mff; upper panel shows wild-type (WT) and mutant-type (MT) Mff 3’UTR sequence diagrams. (I), Transmission electron microscopy observation of mitochondrial ultrastructure in each cell group, scale bar=500 nm. (J), Confocal laser scanning microscopy observation of MFF expression and subcellular localization in each cell group, scale bar=50 μm. #P

Fig. 5 Effect of intervene in SIRT-1 and combine with OA on p62, Beclin-1, LC3B, SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression in HepG2 cells. HepG2 cells were treated with 1.5 mM OA for 48 h, following T6 (2 μM) or CAY (20 μM) 2 h. a The p62, Beclin-1 and LC3B protein expression was detected by Western blotting. b The same method was used to detect the SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression. The bands were normalized using GAPDH. The images were quantified with ImageJ. Data are presented as the mean ± SD; *P < 0.05, **P < 0.01 compared with CON group; #P < 0.05, ##P < 0.01 compared with OA group