SESN2 Antibody - #DF12003

Fig. 6. SESN2 inhibited the activation of TGFβ1/Smad3 signaling induced by THBS1 and mechanical stress. (A) Single-cell RNA-sequencing reveals RNA levels of SESN2 in the non-LFH and LFH groups and the quantification of the expression of the SESN2 in all cells. (B) Immunohistochemical staining of SESN2 of the LF samples from LFH group (n = 6) and non-LFH group (n = 6), and the percentage of positive cells was quantified. Scale bar = 20 μm. (C) Western blotting assay showing protein levels of SESN2 in LF samples of non-LFH and LFH group. (D) Western blotting assay showing protein levels of SESN2 in LF cells which were mechanically stretched for 0 h, 8 h and 16 h. (E) Double-labeling immunohistofluorescence staining of THBS1 and TGFβ1 of cells with different treatments. Nuclei were stained with DAPI (blue). THBS1 was stained green. TGFβ1 was stained red. Scale bar = 20 μm. (F) The qRT-PCR analysis results showing the mRNA levels of THBS1, TGFβ1, COL1A2, and ACTA2 (α-SMA) of LF cells with different treatments. (G) Western blotting assay showing protein levels of THBS1, TGFβ1, COL1A2, ACTA2 (α-SMA), and Smad3 of LF cells with different treatments. These cells were mechanically stretched for 16 h. SESN2 stimulated cells for 24 h. These data are representative of three independent experiments. Error bars: mean ± S.D. ns, not significant.

Fig. 3. PGC-1α silence exacerbated mitochondrial ROS and Sestrin2 and p-S6K1/S6K1 protein decline in the 7β-OHC-treated C2C12 cells. (A) Levels of mitochondrial ROS were measured using MitoSOX Red. Scale = 50 μm. (B) Mean gray value of MitoSOX Red was determined by ImageJ software. (C) Levels of Sestrin2 and p-S6K1/S6K1 protein were measured by western blotting. Three replicates were performed in each experiment. ^p < 0.05, ^^p < 0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)