产品: 磷酸化 LIMK2 (Ser283) 抗体
货号: AF3346
描述: Rabbit polyclonal antibody to Phospho-LIMK2 (Ser283)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 72kDa; 72kD(Calculated).
蛋白号: P53671
RRID: AB_2834761

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-LIMK2 (Ser283) Antibody detects endogenous levels of LIMK2 only when phosphorylated at Serine 283.
RRID:
AB_2834761
引用格式: Affinity Biosciences Cat# AF3346, RRID:AB_2834761.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

LIM containing protein kinase 2b; LIM domain kinase 2; LIM domain kinase 2b; LIM kinase 2b; LIM motif containing protein kinase 2b; LIMK 2b; LIMK-2; Limk2; LIMK2 b; LIMK2_HUMAN; LIMK2b;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P53671 LIMK2_HUMAN:

Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.

描述:
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers.
序列:
MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
91
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P53671 作为底物

Site PTM Type Enzyme
K57 Ubiquitination
K62 Ubiquitination
S149 Phosphorylation
T151 Phosphorylation
S166 Phosphorylation
S168 Phosphorylation
T179 Phosphorylation
S191 Phosphorylation
T210 Phosphorylation
K272 Ubiquitination
T278 Phosphorylation
S283 Phosphorylation O14965 (AURKA) , Q05655 (PRKCD)
S287 Phosphorylation
S289 Phosphorylation
S291 Phosphorylation
K292 Ubiquitination
S293 Phosphorylation
S297 Phosphorylation
S298 Phosphorylation
K300 Ubiquitination
S310 Phosphorylation
S318 Phosphorylation
K347 Ubiquitination
K355 Acetylation
K355 Ubiquitination
K360 Ubiquitination
K371 Ubiquitination
K378 Ubiquitination
K414 Ubiquitination
K428 Ubiquitination
K487 Ubiquitination
T494 Phosphorylation O14965 (AURKA)
T505 Phosphorylation Q5VT25 (CDC42BPA) , O14965 (AURKA) , Q13464 (ROCK1) , O75116 (ROCK2)

翻译修饰 - P53671 作为激酶

Substrate Site Source
P23528 (CFL1) S3 Uniprot
P60981 (DSTN) S3 Uniprot

研究背景

功能:

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

翻译修饰:

Phosphorylated on serine and/or threonine residues by ROCK1.

细胞定位:

Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Spindle.

Cytoplasm. Nucleus.
Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus.

Cytoplasm. Nucleus.
Note: Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.

亚基结构:

Binds ROCK1 and MARF1 (Ref.9,. Interacts with NISCH (By similarity).

蛋白家族:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

文献引用

1). Function of connexin 43 and RhoA/LIMK2/Cofilin signaling pathway in transient changes of contraction and dilation of human umbilical arterial smooth muscle cells. The International Journal of Biochemistry & Cell Biology (PubMed: 36330887) [IF=4.0]

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