产品: 磷酸化 STAT2 (Tyr690) 抗体
货号: AF3342
描述: Rabbit polyclonal antibody to Phospho-STAT2 (Tyr690)
应用: WB IHC IF/ICC
反应: Human, Rat
预测: Pig, Horse, Dog
分子量: 113kDa; 98kD(Calculated).
蛋白号: P52630
RRID: AB_2834757

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Rat
预测:
Pig(82%), Horse(82%), Dog(91%)
克隆:
Polyclonal
特异性:
Phospho-STAT2 (Tyr690) Antibody detects endogenous levels of STAT2 only when phosphorylated at Tyrosine 690.
RRID:
AB_2834757
引用格式: Affinity Biosciences Cat# AF3342, RRID:AB_2834757.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator; ISGF 3; ISGF3; MGC59816; P113; signal transducer and activator of transcription 2 113kD; Signal transducer and activator of transcription 2; STAT113; Stat2; STAT2_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
序列:
MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYECGRCSQDPESLLLQHNLRKFCRDIQPFSQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVESQQHEIESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRRKEVLDASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELKGLSCLVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRLQEGNESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKGPLGVTEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPKAPWSLLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMVSQTVPEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHFYTDGPLMPSDF

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Dog
91
Pig
82
Horse
82
Bovine
70
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P52630 作为底物

Site PTM Type Enzyme
S12 Phosphorylation
T136 Phosphorylation
K158 Acetylation
K158 Ubiquitination
K161 Ubiquitination
K167 Ubiquitination
K184 Ubiquitination
S187 Phosphorylation
K194 Ubiquitination
K197 Ubiquitination
K208 Ubiquitination
K218 Ubiquitination
K239 Ubiquitination
K244 Ubiquitination
K280 Ubiquitination
S283 Phosphorylation
S287 Phosphorylation
T294 Phosphorylation
K295 Ubiquitination
K375 Acetylation
K375 Ubiquitination
K384 Acetylation
K384 Ubiquitination
T387 Phosphorylation
K443 Ubiquitination
K523 Ubiquitination
K543 Ubiquitination
K550 Ubiquitination
S595 Phosphorylation
T597 Phosphorylation
Y631 Phosphorylation
K681 Ubiquitination
Y690 Phosphorylation P06239 (LCK)
S699 Phosphorylation
S734 Phosphorylation
S753 Phosphorylation
T800 Phosphorylation
Y833 Phosphorylation
S849 Phosphorylation

研究背景

功能:

Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively.

翻译修饰:

Tyrosine phosphorylated in response to IFN-alpha. Phosphorylation at Ser-287 negatively regulates the transcriptional response.

'Lys-48'-linked ubiquitination by DCST1 leads to STAT2 proteasomal degradation.

细胞定位:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus upon activation by IFN-alpha/beta.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation (By similarity). The heterodimer STAT1:STAT2 forms the interferon-stimulated gene factor 3 complex (ISGF3) with IRF9; interacts with IRF9 in the cytoplasm (By similarity). Interacts with CRSP2 and CRSP6. Can form a homodimer upon IFN-alpha induced phosphorylation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with ARL2BP (By similarity). Interacts with E3 ubiquitin ligase DCST1; the interaction results in STAT2 ubiquitin-mediated proteasomal degradation.

(Microbial infection) Interacts with vaccinia virus protein C6.

(Microbial infection) Interacts with Simian virus 5 protein V.

(Microbial infection) Interacts with Rabies virus phosphoprotein.

(Microbial infection) Interacts with Human cytomegalovirus/HHV-5 protein UL123; this interaction promotes viral growth.

(Microbial infection) Interacts with Dengue virus NS5; this interaction inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation.

蛋白家族:

Belongs to the transcription factor STAT family.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Huang HX et al. Swine acute diarrhoea syndrome coronavirus (SADS-CoV) Nsp5 antagonizes type I interferon signaling by cleaving DCP1A. Frontiers in Immunology 2023;14(1196031) (PubMed: 37283741) [IF=7.3]

Application: WB    Species: Human    Sample: HEK-293T cells

Figure 4 DCP1A activates the type I IFN signaling pathway. (A) ST, HEK-293T, and Vero-E6 cells were transfected with 2 µg/well empty vector or pXJ40-HA-sDCP1A, pXJ40-HA-hDCP1A, and pXJ40-HA-mDCP1A expression plasmids. After 24 h of transfection, the cells were infected with VSV-GFP for 12 h, and the expression of DCP1A was examined by Western blotting with an anti-HA antibody. Then, the replication of VSV-GFP was analysed via fluorescence microscopy. (B) HEK-293T cells were transfected with pXJ40-HA vector and various amounts of the pXJ40-HA-mDCP1A expression plasmid. (C) pXJ40-HA vector, pXJ40-HA-h-DCP1A expression plasmid, DCP1A siRNA and negative control siRNA were transfected into HEK-293T cells. After 30 h, cells were collected for Western blot analysis. Cells were analyzed by Western blotting with anti-STAT2, anti-phospho STAT2, anti-STAT1, anti-phospho STAT1, anti-IRF3, anti-phospho IRF3, anti-P65, anti-phospho P65, anti-HA, and β-actin antibodies. (D) pXJ40-HA vector, pXJ40-HA-h-DCP1A expression plasmid, DCP1A siRNA and negative control siRNA were transfected into HEK-293T cells. After 30 h, IFN-β mRNA levels were analysed by RT−qPCR. All data are reported as mean±SD. For all experiments, *p < 0.05 and ***p < 0.001 were considered to be statistically significant.

2). Hu Y et al. Genomic distribution of signal transducer and activator of transcription (STAT) family in colorectal cancer. Human Cell 2022 Oct 25; (PubMed: 36284066) [IF=4.3]

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