VEGFR2 Antibody - #AF4726

Figure 3: Daidzein improves migration and proliferation of BMECs. (A) BMECs were cultured and treated with different dose of daidzein (0, 10, 20 µM). Scratch wound healing assay was performed to observe the effect of daidzein on the migration of BMECs. Scale bar, 100 µm. (B) Quantitative analysis of wound healing rate. (C) Representative images of immunofluorescence staining for Ki67 (green) in BMECs treated with different dose of daidzein (0, 10 µM). Nuclei were stained with DAPI (blue). White arrows indicate Ki67+ cells. Scale bar, 100 µm. (D) Quantification of the percentage of Ki67+ nuclei among the total nuclei. (E, F) RNA was isolated from BMECs in the control and daidzein groups, and the mRNA expression of Angpt-1 and Vegf was detected with qRT-PCR. (G) BMECs were cultured and treated with different dose of daidzein, protein was harvested at indicated time points, western blotting was performed to detect the expression and ANGPT-1 phospho-VEGFR and GAPDH was used as reference proteins for data normalization. (H) ANGPT-1 and phosphorylation levels of VEGFR were quantified and normalized to total VEGFR. Data were presented as mean ± SE. *p < 0.05 and **p < 0.01 as determined by One way ANOVA or two-tailed unpaired t-test.