产品: hnRPD 抗体
货号: AF0092
描述: Rabbit polyclonal antibody to hnRPD
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 38kDa; 38kD(Calculated).
蛋白号: Q14103
RRID: AB_2833275

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
hnRPD Antibody detects endogenous levels of total hnRPD.
RRID:
AB_2833275
引用格式: Affinity Biosciences Cat# AF0092, RRID:AB_2833275.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ARE binding protein AUFI type A; AU-rich element RNA-binding protein 1; AUF; AUF1; AUF1A; Heterogeneous nuclear ribonucleoprotein D0; hnRNP D; hnRNP D0; Hnrnpd; hnRNPD0; HNRPD; HNRPD_HUMAN; P37;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
hnRNP D0 a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). Implicated in the regulation of mRNA stability. Has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. Four alternatively spliced transcript variants have been described.
序列:
MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGSAESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQGYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14103 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S71 Phosphorylation
K72 Acetylation
K72 Ubiquitination
S80 Phosphorylation
S82 Phosphorylation
S83 Phosphorylation P49841 (GSK3B)
S87 Phosphorylation P17612 (PRKACA)
T91 Phosphorylation
R94 Methylation
S105 Phosphorylation
K110 Acetylation
K110 Ubiquitination
K111 Acetylation
K111 Ubiquitination
K114 Acetylation
K114 Ubiquitination
K119 Acetylation
K119 Ubiquitination
C126 S-Nitrosylation
T127 Phosphorylation
K129 Acetylation
K129 Ubiquitination
T134 Phosphorylation
S137 Phosphorylation
R138 Methylation
K153 Acetylation
K153 Ubiquitination
K158 Acetylation
K165 Acetylation
K165 Ubiquitination
K183 Ubiquitination
S190 Phosphorylation
T193 Phosphorylation P24941 (CDK2)
K197 Acetylation
K197 Sumoylation
K197 Ubiquitination
Y201 Phosphorylation
S210 Phosphorylation
K218 Acetylation
K218 Methylation
K218 Ubiquitination
C226 S-Nitrosylation
K231 Acetylation
K231 Ubiquitination
K238 Acetylation
K243 Acetylation
K243 Ubiquitination
Y244 Phosphorylation
K251 Acetylation
K251 Ubiquitination
K255 Ubiquitination
K260 Methylation
K260 Ubiquitination
Y263 Phosphorylation
S271 Phosphorylation
R272 Methylation
R278 Methylation
R280 Methylation
R282 Methylation
R345 Methylation
S351 Phosphorylation
K353 Acetylation
K353 Methylation

研究背景

功能:

Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.

翻译修饰:

Arg-345 is dimethylated, probably to asymmetric dimethylarginine.

Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation (By similarity).

细胞定位:

Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Cytoplasmic localization oscillates diurnally.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR and SYNCRIP. Interacts with IGF2BP2. Interacts with GTPBP1. Interacts with EIF4G1; the interaction requires RNA. Interacts with EIF3B and RPS3.

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