5 Lipoxygenase Antibody - #AF4699

FIGURE 7 NDUFA4L2 regulated downstream p38-5-LO pathway may be an important mechanism for hypoxic pulmonary vascular remodelling. A, After interference with NDUFA4L2, Western blotting was used to detect the expression of p-ERK/ERK, p-p38/ p38, p-ERK/ERK in hypoxic HPASMCs; (B) After interference with NDUFA4L2, Western blotting was used to detect the expression of p-5-LO/5-LO in hypoxic HPASMCs. NC, negative control; Nor, normoxia; Hyp, hypoxia. *P < .05, **P < .01. n = 6. All of the values are denoted as mean ± SEM

Fig. 4. TCEA alleviates CS-induced COPD mice disease progression by inhibiting ferroptosis. (A) Immunohistochemical staining of 4-HNE, GPX4 and SLC7A11 in lung sections (scale bar = 100 μm). (B) Quantification results of 4-HNE, GPX4 and SLC7A11 in lung sections (n = 3 for per group, each sample for 8 fields) from a. (C–E) The expression level of GSH, SOD and MDA of lung sections treated with TCEA (n = 3 per group). (F–G) Immunoblotting and quantification analysis of Nrf2, GPX4 and SLC7A11 in mice lung tissue. (H) The mRNA levels of SLC7A11 and GPX4 were measured in lung tissue using qPCR. (I–J) Immunoblotting and quantification analysis of ACSL4, ALOX5 and COX2 in mice lung sections.