Phospho-VASP (Ser157) Antibody - #AF3337

Fig. 4. ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, ERK5, JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of NOX2, p67phox, NOX1, NOXO1 and Rac in WT and p47phox-/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRPstimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.

FIGURE 6 ROS generation, phosphorylation of ERK1/2, p38, AKT and VASP and cGMP level. After matrine treatment, platelet ROS generation was measured using H2DCF-DA by flow cytometry (A). Matrine-treated platelets were stimulated with 5 μg/ml CRP or 1 U/ml thrombin for 5 min to measure the phosphorylation level of ERK1/2, p38, AKT (B and C) or VASP (D) by western blot (mean ± SD, n = 3), cGMP level by ELISA (mean ± SE, n = 3) (E). Compared with 0, * p < 0.05; **p < 0.01; ***p < 0.001.