产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3337, RRID:AB_2834752.
展开/折叠
Vasodilator stimulated phosphoprotein; Vasodilator-stimulated phosphoprotein; VASP; VASP_HUMAN;
抗原和靶标
- P50552 VASP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSETVICSSRATVMLYDDGNKRWLPAGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGVKYNQATPNFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAGGPPAPPAGGPPPPPGPPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAMLARRRKATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSP
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P50552 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Acetylation | Uniprot | |
S2 | Phosphorylation | Uniprot | |
S8 | Phosphorylation | Uniprot | |
Y16 | Phosphorylation | Uniprot | |
Y39 | Phosphorylation | Q9UM73 (ALK) | Uniprot |
T43 | Phosphorylation | Uniprot | |
S46 | Phosphorylation | Uniprot | |
R68 | Methylation | Uniprot | |
K71 | Ubiquitination | Uniprot | |
S134 | Phosphorylation | Uniprot | |
S157 | Phosphorylation | P17252 (PRKCA) , P17612 (PRKACA) , Q15139 (PRKD1) , Q13976 (PRKG1) , Q9BZL6 (PRKD2) | Uniprot |
S239 | Phosphorylation | Q13976 (PRKG1) , Q13237 (PRKG2) , P17612 (PRKACA) , Q9BZL6 (PRKD2) | Uniprot |
T249 | Phosphorylation | Uniprot | |
K252 | Acetylation | Uniprot | |
S258 | Phosphorylation | Uniprot | |
T278 | Phosphorylation | Q15418 (RPS6KA1) , P54646 (PRKAA2) , P17612 (PRKACA) , Q13131 (PRKAA1) , Q13976 (PRKG1) | Uniprot |
K283 | Acetylation | Uniprot | |
T284 | Phosphorylation | Uniprot | |
K286 | Sumoylation | Uniprot | |
S289 | Phosphorylation | Uniprot | |
S305 | Phosphorylation | Uniprot | |
K312 | Ubiquitination | Uniprot | |
S314 | Phosphorylation | Uniprot | |
T315 | Phosphorylation | Uniprot | |
T316 | Phosphorylation | Uniprot | |
K321 | Methylation | Uniprot | |
K321 | Ubiquitination | Uniprot | |
S322 | Phosphorylation | P54646 (PRKAA2) , Q9BZL6 (PRKD2) , Q15139 (PRKD1) | Uniprot |
S323 | Phosphorylation | Uniprot | |
S324 | Phosphorylation | Uniprot | |
S325 | Phosphorylation | Uniprot | |
T327 | Phosphorylation | Uniprot | |
T328 | Phosphorylation | Uniprot | |
T331 | Phosphorylation | Uniprot | |
T335 | Phosphorylation | Uniprot | |
S337 | Phosphorylation | Uniprot | |
Y341 | Phosphorylation | Uniprot | |
K348 | Ubiquitination | Uniprot | |
K363 | Ubiquitination | Uniprot |
研究背景
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation at Ser-157 by PKA is required for localization to the tight junctions in epithelial cells. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Phosphorylation at Ser-322 by AMPK also alters actin filament binding. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
Cytoplasm. Cytoplasm>Cytoskeleton. Cell junction>Focal adhesion. Cell junction>Tight junction. Cell projection>Lamellipodium membrane. Cell projection>Filopodium membrane.
Note: Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes).
Highly expressed in platelets.
Homotetramer. Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1. Interacts, via the EVH1 domain, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells. Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA. Interacts with APBB1IP. Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP (By similarity). Interacts weakly with MEFV.
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
The WH1 domain mediates interaction with XIRP1.
Belongs to the Ena/VASP family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
文献引用
Application: WB Species: Human Sample: platelets
Application: WB Species: Human Sample:
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