Acetyl-Histone H3 (Lys9/14/18/23/27) Antibody - #AF4365

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产品: 乙酰化Histone H3 (Lys9/14/18/23/27) 抗体
货号: AF4365
描述: Rabbit polyclonal antibody to Acetyl-Histone H3 (Lys9/14/18/23/27)
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Bovine
蛋白号: P68431 | Q71DI3 | P84243
RRID: AB_2844627

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Acetyl-Histone H3 (Lys9/14/18/23/27) Antibody detects endogenous levels of Acetyl-Histone H3 only when acetylated at Lys9/14/18/23/27.
RRID:
AB_2844627
引用格式: Affinity Biosciences Cat# AF4365, RRID:AB_2844627.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

H3 histone family, member A; H3/A; H31_HUMAN; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; Histone cluster 1, H3a; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; ;H3.3A; HIST1 cluster, H3E; H3 histone family, member A; H3.1; H3/l; H3F3; H3FF; H3FJ; H3FL; Histone gene cluster 1, H3 histone family, member E; histone H3.1t; Histone H3/o; FLJ92264; H 3; H3; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member F; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member T; H3 histone, family 3A; H3/A; H3/b; H3/c; H3/d; h3/f; H3/h; H3/i; H3/j; H3/k; H3/t; H31_HUMAN; H3F1K; H3F3A; H3FA; H3FB; H3FC; H3FD; H3FH; H3FI; H3FK; HIST1 cluster, H3A; HIST1 cluster, H3B; HIST1 cluster, H3C; HIST1 cluster, H3D; HIST1 cluster, H3F; HIST1 cluster, H3G; HIST1 cluster, H3H; HIST1 cluster, H3I; HIST1 cluster, H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; Histone 1, H3a; Histone 1, H3b; Histone 1, H3c; Histone 1, H3d; Histone 1, H3e; Histone 1, H3f; Histone 1, H3g; Histone 1, H3h; Histone 1, H3i; Histone 3, H3; histone cluster 1 H3 family member a; histone cluster 1 H3 family member b; histone cluster 1 H3 family member c; histone cluster 1 H3 family member d; histone cluster 1 H3 family member e; histone cluster 1 H3 family member f; histone cluster 1 H3 family member g; histone cluster 1 H3 family member h; histone cluster 1 H3 family member i; histone cluster 1 H3 family member j; Histone cluster 1, H3a; Histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3d; Histone cluster 1, H3e; Histone cluster 1, H3f; Histone cluster 1, H3g; Histone cluster 1, H3i; Histone cluster 1, H3j; Histone gene cluster 1, H3 histone family, member A; Histone gene cluster 1, H3 histone family, member B; Histone gene cluster 1, H3 histone family, member C; Histone gene cluster 1, H3 histone family, member D; Histone gene cluster 1, H3 histone family, member F; Histone gene cluster 1, H3 histone family, member G; Histone gene cluster 1, H3 histone family, member H; Histone gene cluster 1, H3 histone family, member I; Histone gene cluster 1, H3 histone family, member J; Histone gene cluster 1, H3A; Histone gene cluster 1, H3B; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3E; Histone gene cluster 1, H3F; Histone gene cluster 1, H3G; Histone gene cluster 1, H3H; Histone gene cluster 1, H3I; Histone gene cluster 1, H3J; Histone H 3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; H3 histone family 3A; H3 histone family 3B; H3 histone, family 3B (H3.3B); H3.3; H3.3A; H3.3B; H33_HUMAN; H3F3; H3F3A; H3f3b; Histone H3.3; Histone H3.3Q; Histone H3.A; Histone H3.B; MGC87782; MGC87783;

抗原和靶标

免疫原:

A synthesized peptide derived from human Histone H3 around the acetylation site of Lys9/14/18/23/27.

基因/基因ID:
HIST2H3A; HIST2H3C; HIST2H3D(126961) | HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J(333932) | H3F3A; H3F3B(653604)

研究领域

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

文献引用

1). Lactate drives epithelial-mesenchymal transition in diabetic kidney disease via the H3K14la/KLF5 pathway. Redox biology, 2024 (PubMed: 38925041) [IF=10.7]

Application: WB    Species: Mouse    Sample:

Fig. 5. Histone lactylaction is increased in db/db mice and AGEs-induced mTEC. (A) Immunoblot analysis of Pan kla expression in db/db mice. (B) The Pan kla immunoblots in AGE-induced mTEC. (C) The Pan kla and Pan kac immunoblots in AGE-induced mTEC at 0, 4, 8, 12, 24, and 48 h. (D–E) Immunoblot analysis demonstrating the expression of Pan kla, H3K14la, H3K18la, H3K9la, H3K23la, H3K27la and H3K14ac in AGEs-induced mTEC. (F) Immunofluorescent staining of Pan kla (red) and DAPI (blue). Scale bars, 50 μm. (G–H) Immunofluorescent staining of H3K14la (red) and DAPI (blue). Scale bars, 50 μm. (I–K) Immunoblot analysis of Pan kla and H3K14la in AGEs-induced mTEC. All statistic data were presented as mean ± SEM. Unpaired two tailed t-test. Data are expressed as Mean ± SEM, #P < 0.05, ##P < 0.01; *P < 0.05, **P < 0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
2). Discovery of Highly Potent and Orally Bioavailable Histone Deacetylase 3 Inhibitors as Immunomodulators and Enhancers of DNA-Damage Response in Cancer Therapy. Journal of medicinal chemistry, 2025 (PubMed: 39873221) [IF=6.8]
3). Discovery of Janus Kinase and Histone Deacetylase Dual Inhibitors as a New Strategy to Treat Psoriasis. Journal of medicinal chemistry, 2024 (PubMed: 39415349) [IF=6.8]
4). TrSnt2-mediated histone H3 deacetylation governs mitophagy, ROS accumulation, ergosterol synthesis, and pathogenicity in Trichothecium roseum. Postharvest Biology and Technology, 2024 [IF=6.4]
5). Design, synthesis and bioevaluation of novel hydrazide derivatives as enhancers of immunotherapy and DNA-damage response in antitumor therapy. European journal of medicinal chemistry, 2025 (PubMed: 40233424) [IF=6.0]
6). Antidepressant effect and mechanism of TMP269 on stress-induced depressive-like behavior in mice. Biochemical pharmacology, 2024 (PubMed: 38801927) [IF=5.3]
7). Sodium valproate attenuates the iE-DAP induced inflammatory response by inhibiting the NOD1-NF-κB pathway and histone modifications in bovine mammary epithelial cells. International Immunopharmacology, 2020 (PubMed: 32182568) [IF=4.8]

Application: WB    Species: bovine    Sample: BMECs

Fig. 5. |Protein expression and location of STAT1, HDACs and acetylated histone H3 in iE-DAP-stimulated BMECs after pretreatment with VPA. Western blot analysis showing the expression levels of STAT1 and HDAC1-3 (B), histone H3 and acetylated histone H3 (C) and the ratio of acetylated histone H3 to histone H3 (D).
8). Synthesis and biological evaluation of novel 2,4-dianilinopyrimidine derivatives as potent dual janus kinase 2 and histone deacetylases inhibitors. Journal of Molecular Structure, 2022 [IF=4.0]
9). Synthesis and Biological Evaluation of HDAC Inhibitors With a Novel Zinc Binding Group. Frontiers in Chemistry, 2020 (PubMed: 32351936) [IF=3.8]
10). Sodium butyrate promotes lipopolysaccharide-induced innate immune responses by enhancing mitogen-activated protein kinase activation and histone acetylation in bovine mammary epithelial cells. JOURNAL OF DAIRY SCIENCE, 2020 (PubMed: 33010913) [IF=3.7]

Application: WB    Species:    Sample: macrophages

Figure 7.| Relationship between histone acetylation and the upregulation of proinflammatory cytokines, chemokines, and β-defensin transcription by sodium butyrate (NaB) during LPS stimulation. (A) Bands of the protein related to histone H3 and acetylated histone H3. Quantification of the bands from Western blots.
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