产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3330, RRID:AB_2834745.
展开/折叠
campomelic dysplasia autosomal sex reversal; CMD 1; CMD1; CMPD 1; CMPD1; SOX 9; Sox9; SOX9_HUMAN; SRA 1; SRA1; SRXX2; SRXY10; SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal; SRY (sex determining region Y) box 9; SRY (sex determining region Y)-box 9; SRY (sex-determining region Y)-box 9 protein; SRY related HMG box gene 9; Transcription factor SOX 9; Transcription factor SOX-9; transcription factor SOX9;
抗原和靶标
- P48436 SOX9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQAPPPPPQQPPQAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P48436 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T11 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| K61 | Acetylation | Uniprot | |
| S64 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K68 | Ubiquitination | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| S181 | Phosphorylation | P17612 (PRKACA) , Q13237 (PRKG2) , Q13464 (ROCK1) | Uniprot |
| T196 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K398 | Acetylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| Y442 | Phosphorylation | Uniprot |
研究背景
Transcriptional regulator that plays a role in chondrocytes differentiation and skeletal development. Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219 (By similarity).
Ubiquitinated. Ubiquitination leads to proteasomal degradation and is negatively regulated by DDRGK1.
Nucleus.
Interacts (via C-terminus) with ZNF219. Interacts with DDRGK1.
研究领域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
限制条款
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