Oxytocin Receptor Antibody - #AF4667

Figure 7 Characterization of TLR4, and MAPK/NK-κB activation as well as expression of UAPs (OTR, CX43) in myomentrium of pregnant women before and after the onset of labor at term. Myomentrium were obtained for determination of TLR4 and MAPK/NF-κB activation and the expression of UAPs. (A) The representative sections of TLR4 expression in pregnant women myomentrium at term. Myometrium samples were obtained from healthy pregnant women before and after the onset of labor at term. The tissue sections were used for TLR4 immunocytochemistry analysis. Arrows indicate the positive staining of TLR4. (B, C) The protein level of TLR4 and UAPs was determined by Western blotting analysis. Representative bands were placed on the left of corresponding histogram. (D) The protein level of MAPK/NK-κB was determined by Western blotting analysis. Representative bands were listed on top of histogram. Data were expressed as mean ± SEM. (n= 12). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; P>0.05, not significant.

Fig. 2. WBs with anti-OXTR antibodies in OXTR KO (KO1 and KO2) and WT (WT1 and WT2) mouse brain. OXTR antibody ab181077 (Abcam) (A). OXTR antibody ab217212 (Abcam) (B). OXTR antibody AF4667 (Affinity Biomarker labs) (C). OXTR antibody 23045-1-AP (Proteintech) (D). OXTR antibody FNab06056 (FineTest) (E). OXTR antibody sc-515809 (Santa Cruz Biotechnology) (F). Anti-rabbit igG (HRP) heavy and light chain (H + L) (G) and anti-mouse igG (HRP) heavy and light chain (H + L) (H) in KO and WT mouse brain as negative controls (no primary antibody). Molecular weight marker (kDa) was used to determine the apparent molecular weight. β-actin was used as sample loading control, confirming equal sample loading. WT: Wild Type and KO: Knock Out.

Fig. 4. IHC with anti-OXTR antibodies in uterus tissue from WT and OXTR KO mice. OXTR antibody ab181077 (Abcam) (A). OXTR antibody ab217212 (Abcam) (B). OXTR antibody AF4667 (Affinity Biomarker labs) (C). OXTR antibody 23045-1-AP (Proteintech) (D). OXTR antibody FNab06056 (FineTest) (E). OXTR-Venus mouse uterus showing GFP fluorescence in OXTR positive cells, as positive control (no antibodies) (F). Scale bar: 50 μm. Note that only antibody ab181077, displayed in panel A, shows specific staining. Arrows indicate OXTR positive cells. WT: Wild Type and KO: Knock Out.