P2X7 Antibody - #AF4626

FIGURE 8 Bufalin-PLGA MS suppressed the protein expression of TRPV1 and P2X7 in CCI rats. (A) SDS-PAGE bands of TRPV1 and P2X7 protein in the DRG of different group rats. (B) Expression levels of TRPV1 protein in the DRG of different group rats. (C) Expression level of P2X7 protein in the DRG of different group rats. Data are expressed in the form of mean ± SEM, n = 3. ###p < 0.001 compared to the control group, *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the CCI group.

FIGURE 9 Expressions of TRPV1 and P2X7 were visualized and quantified via immunofluorescence staining. (A) Immunofluorescence detection of TRPV1 protein in the DRG; green and blue staining represent TRPV1 and DAPI, respectively. (B) Immunofluorescence detection of P2X7 protein in the DRG; green and blue staining represent P2X7 and DAPI, respectively. Data is expressed in the form of mean ± SEM, n = 3. ###p < 0.001 compared to the control group, *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the CCI group. (Scale bar = 20 µm).

Figure 4 Positive expression of P2X7R, NLRP3, and GSDMD in each group (immunofluorescence staining, × 400). (A) Representative immunofluorescence images of P2X7R in the brain cortex. (B) Representative immunofluorescence images of NLRP3 in the brain cortex. (C) Representative immunofluorescence images of GSDMD in the brain cortex. (D) P2X7R positive expression per site. (E) NLRP3 positive expression per site. (F) GSDMD positive expression per site. Sham: Vascular isolation surgery-no ligation; I/R: Modeled without any treatment; EA: Electroacupuncture-treated model rats. BBG: P2X7R inhibitor injection (50 mg/kg); MCC950: NLRP3 inhibitor injection (50 mg/kg). P2X7R: purinergic P2X receptor 7; GSDMD: gasdermin D; NLRP3: NOD-like receptor protein 3. Data are presented as mean ± standard error of the mean (n = 3). Compared to the Sham group, **P

Figure 5 Protein and mRNA expression of pyroptosis-related proteins. (A) Representative images of protein bands of ASC, Caspase-1, P2X7R, NLRP3, and GSDMD. B-F: ASC (B), Caspase-1 (C), P2X7R (D), NLRP3 (E), and GSDMD (F) quantitative analysis showing the protein levels. G-I: P2X7R (G), NLRP3 (H), and GSDMD (I) quantitative analysis showing the mRNA levels. Sham: Vascular isolation surgery-no ligation; I/R: Modeled without any treatment; EA: Electroacupuncture-treated model rats. BBG: P2X7R inhibitor injection (50 mg/kg); MCC950: NLRP3 inhibitor injection (50 mg/kg). P2X7R: purinergic P2X receptor 7; GSDMD: gasdermin D; NLRP3: NOD-like receptor protein 3. Data are presented as mean ± standard error of mean (n = 3). Compared to the Sham group, **P