Phospho-PERK (Ser1096) Antibody - #AF4499

>>买2送1 已结束>>
>>赠JD卡 已结束>>
(7)   (15)  
产品: 磷酸化 PERK (Ser1096) 抗体
货号: AF4499
描述: Rabbit polyclonal antibody to Phospho-PERK (Ser1096)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Rabbit, Dog, Xenopus
蛋白号: Q9NZJ5
RRID: AB_2844550

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1500 现货
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-PERK (Ser1096) Antibody detects endogenous levels of PERK only when phosphorylated at S1096.
RRID:
AB_2844550
引用格式: Affinity Biosciences Cat# AF4499, RRID:AB_2844550.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp781H1925; E2AK3_HUMAN; EC 2.7.11.1; Eif2ak3; Eukaryotic translation initiation factor 2 alpha kinase 3; Eukaryotic translation initiation factor 2-alpha kinase 3; Heme regulated EIF2 alpha kinase; HRI; HsPEK; Pancreatic eIF2 alpha kinase; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; PRKR-like endoplasmic reticulum kinase; WRS;

抗原和靶标

免疫原:

A synthesized peptide derived from human perk around the phosphorylation site of S1096.

基因/基因ID:
EIF2AK3(9451)

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

文献引用

1). Palmatine activation of TFEB enhances autophagy and alleviates endoplasmic reticulum stress in intervertebral disc degeneration. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2025 (PubMed: 39933468) [IF=6.7]
2). Acute nitrite exposure-induced oxidative damage, endoplasmic reticulum stress, autophagy and apoptosis caused gill tissue damage of grass carp (Ctenopharyngodon idella): Relieved by dietary protein. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35994904) [IF=6.2]

Application: WB    Species: Fish    Sample: gill tissue

Fig. 5. Changes in related protein levels in grass carp gill fed with different protein levels and exposed to nitrite for 96 h. Data represent the means of each group. Treatment 1 of the control group was used as a reference. NS: not significant.
3). The role of vitamin E in polyunsaturated fatty acid synthesis and alleviating endoplasmic reticulum stress in sub-adult grass carp (Ctenopharyngodon idella). Animal nutrition (Zhongguo xu mu shou yi xue hui), 2024 (PubMed: 38371478) [IF=6.1]

Application: WB    Species: fish    Sample:

Fig. 5 Effects of vitamin E on the relative protein expressions of endoplasmic reticulum stress-related proteins in the muscle of sub-adult grass carp. (A) Western blots for p-PERK (Ser1096), p-IRE1 (Ser724), ATF6 and GRP78. Quantification of (B) p-PERK (Ser1096), (C) p-IRE1 (Ser724), (D) ATF6, and (E) GRP78 normalized to β-actin. Results are represented as the mean ± SD. The data are the means of three replicates with two fishes per replicate (n = 3). Mean values within the same row with different superscripts are significantly different (P < 0.05). p-PERK (Ser1096) = protein kinase R-like endoplasmic reticulum kinase (phospho-Ser1096); p-IRE1 (Ser724) = inositol-requiring enzyme 1 (phospho-Ser724); ATF6 = activating transcription factor 6; GRP78 = glucose regulatory protein 78.
4). CTRP1 attenuates cerebral ischemia/reperfusion injury via the PERK signaling pathway. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34422821) [IF=4.6]

Application: WB    Species: Rat    Sample: brain tissue

FIGURE 5 CTRP1 inhibited ERS via PERK signal pathway in the cortex of MCAO/R-treated rats. (A) Western blot analyzed the expression of PERK, p-PERK, GRP78, ATF6, ATF4, IRE1α, p-IRE1α. n = 4 per group. (B) CTRP1 affected the interaction between PERK and GRP78 after CIRI. n = 3 per group. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 vs. sham group, ####p < 0.0001, ###p < 0.001, ## p < 0.01, #p < 0.05 vs. MCAO/R + LV-NC group.
5). Baicalein modulates endoplasmic reticulum stress by activating SIRT3 to attenuate the dysfunction of retinal microvascular endothelial cells under high glucose conditions. Experimental eye research, 2025 (PubMed: 39920974) [IF=3.0]
6). Suberoylanilide hydroxamic acid upregulates histone acetylation and activates endoplasmic reticulum stress to induce apoptosis in HepG2 liver cancer cells. Oncology Letters, 2019 (PubMed: 31516571) [IF=2.5]

Application: WB    Species: human    Sample: HepG2 cells

Figure 3. |SAHA induces ER stress in HepG2 cells. (A) HepG2 cells were treated with SAHA at the indicated concentrations for 48 h, and the mRNA levels of endoplasmic reticulum stress associated genes (GRP78, PERK, ATF4 and CHOP) were analyzed by reverse transcription quantitative PCR. (B and C) Representative images (one of three experiments) showing the (B) western blot analysis of GRP78, PERK, p‑PERK, ATF4 and CHOP protein expression in HepG2 cells exposed to SAHA at the indicated concentrations and (C) their relative protein expression levels.
7). Raf/MEK/ERK Signaling Pathway Is Involved in the Inhibition of Glioma Cell Proliferation and Invasion in the Ketogenic Microenvironment. Current medical science, 2023 (PubMed: 37498407) [IF=2.0]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.