| 产品: | 磷酸化 MERIT40 (Ser29) 抗体 |
| 货号: | AF4498 |
| 描述: | Rabbit polyclonal antibody to Phospho-MERIT40 (Ser29) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus |
| 分子量: | 40kDa; 37kD(Calculated). |
| 蛋白号: | Q9NWV8 |
| RRID: | AB_2844549 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4498, RRID:AB_2844549.
展开/折叠
BABA1_HUMAN; babam1; BRCA1 A complex subunit MERIT40; BRISC and BRCA1-A complex member 1; C19orf62; FLJ20571; HSPC142; hypothetical protein LOC29086; Mediator of Rap80 interactions and targeting 40 kDa; Mediator of RAP80 interactions and targeting subunit of 40 kDa; MERIT 40; MERIT40; NBA1; New component of the BRCA1 A complex; New component of the BRCA1-A complex; Uncharacterized protein C19orf62;
抗原和靶标
- Q9NWV8 BABA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVAEPSSPTEEEEEEEEHSAEPRPRTRSNPEGAEDRAVGAQASVGSRSEGEGEAASADDGSLNTSGAGPKSWQVPPPAPEVQIRTPRVNCPEKVIICLDLSEEMSLPKLESFNGSKTNALNVSQKMIEMFVRTKHKIDKSHEFALVVVNDDTAWLSGLTSDPRELCSCLYDLETASCSTFNLEGLFSLIQQKTELPVTENVQTIPPPYVVRTILVYSRPPCQPQFSLTEPMKKMFQCPYFFFDVVYIHNGTEEKEEEMSWKDMFAFMGSLDTKGTSYKYEVALAGPALELHNCMAKLLAHPLQRPCQSHASYSLLEEEDEAIEVEATV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NWV8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | P31749 (AKT1) | Uniprot |
| R37 | Methylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| T252 | Phosphorylation | Uniprot |
研究背景
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. In these 2 complexes, it is probably required to maintain the stability of BABAM2 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination.
Cytoplasm. Nucleus.
Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).
Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with ABRAXAS1 and BABAM2. Component of the BRISC complex, at least composed of ABRAXAS2, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Identified in a complex with SHMT2 and the other subunits of the BRISC complex.
The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
Belongs to the BABAM1 family.
研究领域
· Genetic Information Processing > Replication and repair > Homologous recombination.
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