Phospho-PAK1 /PAK2 (Thr423/Thr402) Antibody - #AF4463

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产品: 磷酸化 PAK1 /PAK2 (Thr423/Thr402) 抗体
货号: AF4463
描述: Rabbit polyclonal antibody to Phospho-PAK1 /PAK2 (Thr423/Thr402)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q13153 | Q13177
RRID: AB_2844520

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-PAK1 /PAK2 (Thr423/Thr402) Antibody detects endogenous levels of PAK1 /PAK2 only when phosphorylated at Thr423.
RRID:
AB_2844520
引用格式: Affinity Biosciences Cat# AF4463, RRID:AB_2844520.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADRB2; Alpha PAK; Alpha-PAK; MGC130000; MGC130001; p21 activated kinase 1; p21 protein (Cdc42/Rac) activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p65 PAK; p65-PAK; P68-PAK; PAK alpha; PAK-1; Pak1; PAK1_HUMAN; Paka; PAKalpha; Protein kinase MUK2; Rac/p21-activated kinase; Serine/threonine-protein kinase PAK 1; STE20 homolog yeast; C-t-PAK2; CB422; EC 2.7.11.1; Gamma PAK; Gamma-PAK; hPAK65; Kinase; p21 (CDKN1A) activated kinase 2; p21 (CDKN1A)-activated kinase 2a; p21 activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein Cdc42 Rac activated kinase 2; p21-activated kinase 2; p21-activated kinase, 65-KD; p21-activated protein kinase I; p21CDKN1A activated kinase 2; p27; p34; p58; p65PAK; PAK 2; PAK-2; PAK-2p34; Pak2; PAK2_HUMAN; PAK65; PAKgamma; S6 H4 kinase; S6/H4 kinase; Serine threonine protein kinase PAK 2; Serine/threonine protein kinase PAK 2;

抗原和靶标

免疫原:

A synthesized peptide derived from human PAK1 around the phosphorylation site of Thr423.

基因/基因ID:
PAK1(5058) | PAK2(5062)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

文献引用

1). Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway. Frontiers in Pharmacology, 2022 (PubMed: 35431972) [IF=5.6]

Application: WB    Species: Mouse    Sample: Schwann cells

FIGURE 5 P-bFGF activated the PAK1 pathway to promote autophagy of SCs. (A) Representative western blot images of PAK1, p-PAK1, P62, ATG5, Beclin1 and LC3B expression. (B) Quantification of the levels of PAK1 protein; H2O2 group vs. P-bFGF group: *p < 0.05, P-bFGF group vs. IPA-3 group: **p < 0.01. (C) Quantification of the levels of p-PAK1 protein; H2O2 group vs. P-bFGF group: *p < 0.05, P-bFGF group vs. IPA-3 group: *p < 0.05. (D) Quantification of the levels of P62 protein; H2O2 group vs. P-bFGF group: *p < 0.05, P-bFGF group vs. IPA-3 group: ***p < 0.001. (E) Quantification of the levels of ATG5 protein; H2O2 group vs. P-bFGF group: **p < 0.01, P-bFGF group vs. IPA-3 group: *p < 0.05. (F) Quantification of the levels of the Beclin1 protein; H2O2 group vs. P-bFGF group: ***p < 0.001, P-bFGF group vs. IPA-3 group: **p < 0.01. (G) Quantification of the levels of the LC3B-II protein; H2O2 group vs. P-bFGF group: ***p < 0.001, P-bFGF group vs. IPA-3 group: *p < 0.05. (H) Representative western blot images of P62 and LC3B expression. (I) Quantification of the levels of the P62 protein; H2O2+Baf group vs. H2O2+bFGF + Baf group: *p < 0.05, H2O2+bFGF + Baf + IPA-3 group vs. H2O2+bFGF + IPA-3 group: **p < 0.01. (J) Quantification of the levels of the LC3B-II protein; H2O2+Baf group vs. H2O2+bFGF + Baf group: *p < 0.05, H2O2+bFGF + Baf group vs. H2O2+bFGF + Baf + IPA-3 group: **p < 0.01. The data above were expressed as mean ± SEM (n = 3). A dashed line at 1.0 represented control group.
2). NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway. Gynecologic Oncology, 2019 (PubMed: 30442385) [IF=4.5]

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