Phospho-SYK (Tyr348) Antibody - #AF3314

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产品: 磷酸化 SYK (Tyr348) 抗体
货号: AF3314
描述: Rabbit polyclonal antibody to Phospho-SYK (Tyr348)
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken
蛋白号: P43405
RRID: AB_2834733

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:100-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Phospho-SYK (Tyr348) Antibody detects endogenous levels of SYK only when phosphorylated at Tyrosine 348.
RRID:
AB_2834733
引用格式: Affinity Biosciences Cat# AF3314, RRID:AB_2834733.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 2.7.10.2; kinase Syk; KSYK; KSYK_HUMAN; p72-Syk; p72syk; Spleen tyrosine kinase; Syk; Tyrosine protein kinase SYK; Tyrosine-protein kinase SYK;

抗原和靶标

免疫原:

A synthesized peptide derived from human SYK around the phosphorylation site of Tyr348.

基因/基因ID:
SYK(6850)
描述:
Syk is a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

文献引用

1). The FcεRI signaling pathway is involved in the pathogenesis of lacrimal gland benign lymphoepithelial lesions as shown by transcriptomic analysis. Scientific Reports, 2021 (PubMed: 34750466) [IF=3.8]

Application: IHC    Species: Human    Sample: LGBLEL tissues and orbital CH tissues

Figure 4 Immunohistochemical staining of proteins related to the FcεRI signaling pathway in LGBLEL and CH. P-Syk, P-p38, and P-ERK proteins are shown as brownish yellow in LGBLEL tissues (SP method, magnification X200), and the expression levels were higher than in orbital CH (SP method, magnification X100). P-JNK and P-PI3K were positively expressed in interstitial tissue, but not in lymphoepithelial lesions in LGBLEL (SP method, magnification X200).

Application: WB    Species: Human    Sample: LGBLEL tissues and orbital CH tissues

Figure 5 Comparison of protein immunoblotting and protein content between LGBLEL and orbital CH. (A) Western blotting showed higher expression of proteins related to the FcεRI signaling pathway in LGBLEL than in orbital CH. (B) The protein contents of p-SYK, p-p38, p-JNK, p-PI3K, and p-ERK were significantly higher than in orbital CH (P = 0.0169; P = 0.0074; P = 0.0046; P = 0.0157; P = 0.0156, respectively).

Application: IHC    Species: Human    Sample: LGBLEL tissues

Figure 4 Immunohistochemical staining of proteins related to the FcεRI signaling pathway in LGBLEL and CH. P-Syk, P-p38, and P-ERK proteins are shown as brownish yellow in LGBLEL tissues (SP method, magnification X200), and the expression levels were higher than in orbital CH (SP method, magnification X100). P-JNK and P-PI3K were positively expressed in interstitial tissue, but not in lymphoepithelial lesions in LGBLEL (SP method, magnification X200).

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