产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3312, RRID:AB_2834731.
展开/折叠
70 kDa zeta associated protein; 70 kDa zeta-associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; Selective T cell defect; SRK; STD; Syk related tyrosine kinase; Syk-related tyrosine kinase; Truncated ZAP kinase; Tyrosine protein kinase ZAP70; Tyrosine-protein kinase ZAP-70; TZK; ZAP 70; ZAP70; ZAP70_HUMAN; Zeta chain associated protein kinase 70kD; Zeta chain associated protein kinase 70kDa; Zeta chain associated protein kinase 70kDa isoform 1; Zeta chain associated protein kinase 70kDa isoform 2; Zeta chain TCR associated protein kinase 70kD; Zeta chain TCR associated protein kinase 70kDa;
抗原和靶标
Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
- P43403 ZAP70_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P43403 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Y12 | Phosphorylation | Uniprot | |
K25 | Ubiquitination | Uniprot | |
Y46 | Phosphorylation | Uniprot | |
Y69 | Phosphorylation | P06239 (LCK) | Uniprot |
K75 | Ubiquitination | Uniprot | |
Y87 | Phosphorylation | Uniprot | |
K100 | Ubiquitination | Uniprot | |
S106 | Phosphorylation | Uniprot | |
Y126 | Phosphorylation | P43403 (ZAP70) , P06239 (LCK) | Uniprot |
K132 | Ubiquitination | Uniprot | |
K151 | Ubiquitination | Uniprot | |
Y164 | Phosphorylation | Uniprot | |
S166 | Phosphorylation | Uniprot | |
S167 | Phosphorylation | Uniprot | |
K176 | Ubiquitination | Uniprot | |
Y178 | Phosphorylation | P06239 (LCK) | Uniprot |
S179 | Phosphorylation | Uniprot | |
K186 | Ubiquitination | Uniprot | |
K193 | Ubiquitination | Uniprot | |
T197 | Phosphorylation | Uniprot | |
Y198 | Phosphorylation | Uniprot | |
Y204 | Phosphorylation | Uniprot | |
K206 | Ubiquitination | Uniprot | |
Y209 | Phosphorylation | Uniprot | |
Y211 | Phosphorylation | Uniprot | |
K217 | Ubiquitination | Uniprot | |
K220 | Ubiquitination | Uniprot | |
Y221 | Phosphorylation | Uniprot | |
K242 | Ubiquitination | Uniprot | |
Y248 | Phosphorylation | Uniprot | |
K251 | Ubiquitination | Uniprot | |
S257 | Phosphorylation | Uniprot | |
S258 | Phosphorylation | Uniprot | |
S263 | Phosphorylation | Uniprot | |
T278 | Phosphorylation | Uniprot | |
T286 | Phosphorylation | Uniprot | |
S289 | Phosphorylation | Uniprot | |
Y292 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) | Uniprot |
T293 | Phosphorylation | Uniprot | |
T300 | Phosphorylation | Uniprot | |
S301 | Phosphorylation | Uniprot | |
K304 | Ubiquitination | Uniprot | |
T312 | Phosphorylation | Uniprot | |
S313 | Phosphorylation | Uniprot | |
Y315 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) | Uniprot |
S317 | Phosphorylation | Uniprot | |
Y319 | Phosphorylation | P00519 (ABL1) , P43403 (ZAP70) , P06239 (LCK) | Uniprot |
S320 | Phosphorylation | Uniprot | |
K326 | Ubiquitination | Uniprot | |
K329 | Ubiquitination | Uniprot | |
S351 | Phosphorylation | Uniprot | |
K361 | Ubiquitination | Uniprot | |
K362 | Ubiquitination | Uniprot | |
K369 | Ubiquitination | Uniprot | |
K372 | Ubiquitination | Uniprot | |
K377 | Ubiquitination | Uniprot | |
Y397 | Phosphorylation | Uniprot | |
K450 | Ubiquitination | Uniprot | |
Y451 | Phosphorylation | Uniprot | |
K455 | Ubiquitination | Uniprot | |
Y474 | Phosphorylation | P06239 (LCK) | Uniprot |
K476 | Ubiquitination | Uniprot | |
K484 | Ubiquitination | Uniprot | |
S491 | Phosphorylation | Uniprot | |
Y492 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) | Uniprot |
Y493 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) | Uniprot |
T494 | Phosphorylation | Uniprot | |
S497 | Phosphorylation | Uniprot | |
K500 | Ubiquitination | Uniprot | |
K504 | Ubiquitination | Uniprot | |
Y506 | Phosphorylation | Uniprot | |
S520 | Phosphorylation | Uniprot | |
S534 | Phosphorylation | Uniprot | |
Y535 | Phosphorylation | Uniprot | |
K538 | Ubiquitination | Uniprot | |
K544 | Ubiquitination | Uniprot | |
K556 | Ubiquitination | Uniprot | |
Y569 | Phosphorylation | Uniprot | |
Y597 | Phosphorylation | Uniprot | |
Y598 | Phosphorylation | Uniprot | |
S599 | Phosphorylation | Uniprot | |
K603 | Acetylation | Uniprot | |
K603 | Ubiquitination | Uniprot | |
T611 | Phosphorylation | Uniprot | |
K613 | Ubiquitination | Uniprot |
翻译修饰 - P43403 作为激酶
Substrate | Site | Source |
---|---|---|
O43561-4 (LAT) | Y126 | Uniprot |
O43561-2 (LAT) | Y127 | Uniprot |
O43561-4 (LAT) | Y131 | Uniprot |
O43561-2 (LAT) | Y132 | Uniprot |
O43561-1 (LAT) | Y156 | Uniprot |
O43561 (LAT) | Y161 | Uniprot |
O43561-3 (LAT) | Y163 | Uniprot |
O43561-3 (LAT) | Y168 | Uniprot |
O43561-4 (LAT) | Y170 | Uniprot |
O43561-2 (LAT) | Y171 | Uniprot |
O43561-4 (LAT) | Y190 | Uniprot |
O43561-2 (LAT) | Y191 | Uniprot |
O43561-1 (LAT) | Y200 | Uniprot |
O43561-3 (LAT) | Y207 | Uniprot |
O43561-1 (LAT) | Y220 | Uniprot |
O43561-4 (LAT) | Y225 | Uniprot |
O43561-2 (LAT) | Y226 | Uniprot |
O43561-3 (LAT) | Y227 | Uniprot |
O43561 (LAT) | Y255 | Uniprot |
O43561-3 (LAT) | Y262 | Uniprot |
P15941-7 (MUC1) | Y229 | Uniprot |
P15941-11 (MUC1) | Y238 | Uniprot |
P15941 (MUC1) | Y1203 | Uniprot |
P19174 (PLCG1) | Y783 | Uniprot |
P19174 (PLCG1) | Y1253 | Uniprot |
P29353-7 (SHC1) | Y239 | Uniprot |
P29353-2 (SHC1) | Y240 | Uniprot |
P29353-2 (SHC1) | Y317 | Uniprot |
P29353 (SHC1) | Y349 | Uniprot |
P29353 (SHC1) | Y350 | Uniprot |
P29353 (SHC1) | Y427 | Uniprot |
P43403-2 (ZAP70) | Y8 | Uniprot |
P43403-2 (ZAP70) | Y12 | Uniprot |
P43403 (ZAP70) | Y126 | Uniprot |
P43403 (ZAP70) | Y292 | Uniprot |
P43403 (ZAP70) | Y315 | Uniprot |
P43403 (ZAP70) | Y319 | Uniprot |
P43403 (ZAP70) | Y492 | Uniprot |
P43403 (ZAP70) | Y493 | Uniprot |
P51452 (DUSP3) | Y38 | Uniprot |
P51452 (DUSP3) | Y138 | Uniprot |
Q13094 (LCP2) | Y113 | Uniprot |
Q13094 (LCP2) | Y128 | Uniprot |
Q13094 (LCP2) | Y145 | Uniprot |
Q13094 (LCP2) | Y423 | Uniprot |
Q13094 (LCP2) | Y426 | Uniprot |
Q16539 (MAPK14) | Y323 | Uniprot |
Q8N1K5 (THEMIS) | Y540 | Uniprot |
Q8N1K5 (THEMIS) | Y541 | Uniprot |
Q9UJU6 (DBNL) | Y334 | Uniprot |
Q9UJU6-2 (DBNL) | Y335 | Uniprot |
Q9UJU6 (DBNL) | Y344 | Uniprot |
Q9UJU6-2 (DBNL) | Y345 | Uniprot |
Q9UQC2 (GAB2) | Y614 | Uniprot |
Q9UQQ2 (SH2B3) | Y273 | Uniprot |
研究背景
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22.
Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.
Cytoplasm. Cell membrane>Peripheral membrane protein.
Note: In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity).
Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
Interacts with CD247/CD3Z; this interaction docks ZAP70 at the stimulated TCR. Interacts with NFAM1. Interacts with adapter protein SLA; this interaction negatively regulates T-cell receptor signaling. Interacts with FCRL3. Interacts with VAV1. Interacts with CBL; this interaction promotes ubiquitination, internalization and subsequent degradation of CD247/CD3Z. Identified in a complex with CBL and UBE2L3. Interacts with SHB. Interacts with adapter protein SLA2; this interaction negatively regulates T-cell receptor signaling. Interacts with CBLB. Interacts (via SH2 domains) with RHOH; this interaction regulates ZAP70 subcellular localization. Interacts with DEF6 (By similarity). Interacts (ubiquitinated form) with OTUD7B and UBASH3B.
Composed of 2 N-terminal SH2 domains and a C-terminal kinase domain. The tandem SH2 domains bind to the doubly phosphorylated tyrosine-based activation motif (ITAM) of CD247/CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH (By similarity). The interdomain B located between the second SH2 and the kinase domain contains 3 tyrosines (Tyr-292, Tyr-315, Tyr-319) that are phosphorylated following TCR activation. These sites have been implicated in binding to other signaling molecules including CBL or VAV1. Thus, ZAP70 can also function as a scaffold by recruiting additional factors to the stimulated TCR complex.
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
文献引用
Application: WB Species: mouse Sample: spleen
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