Phospho-CAD (Ser1859) Antibody - #AF4415

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产品: 磷酸化 CAD (Ser1859) 抗体
货号: AF4415
描述: Rabbit polyclonal antibody to Phospho-CAD (Ser1859)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 240kDa; 243kD(Calculated).
蛋白号: P27708
RRID: AB_2844480

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
Phospho-CAD (Ser1859) Antibody detects endogenous levels of CAD only when phosphorylated at Ser1859.
RRID:
AB_2844480
引用格式: Affinity Biosciences Cat# AF4415, RRID:AB_2844480.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Aspartate carbamoyltransferase; Aspartate transcarbamylase; CAD protein; CAD trifunctional protein; Carbamoyl phosphate synthetase 2; CPSase ATCase DHOase; Dihydroorotase; Glutamine dependent carbamoyl phosphate synthase; Multifunctional protein CAD;

抗原和靶标

免疫原:
Uniprot:

P27708(PYR1_HUMAN) >>Visit HPA database.

基因/基因ID:
CAD(790)
序列:
MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRQTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
91
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P27708 作为底物

Site PTM Type Enzyme
A2 Acetylation
S10 Phosphorylation
K127 Ubiquitination
S133 Phosphorylation
K137 Ubiquitination
K165 Ubiquitination
T166 Phosphorylation
T172 Phosphorylation
R177 Methylation
K186 Acetylation
K186 Methylation
K186 Ubiquitination
Y267 Phosphorylation
T375 Phosphorylation
K424 Ubiquitination
T456 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
T475 Phosphorylation
K490 Ubiquitination
T504 Phosphorylation
T585 Phosphorylation
S586 Phosphorylation
K592 Ubiquitination
K598 Ubiquitination
Y602 Phosphorylation
K696 Ubiquitination
Y735 Phosphorylation
K739 Ubiquitination
K747 Acetylation
K747 Ubiquitination
K754 Ubiquitination
K760 Ubiquitination
S761 Phosphorylation
K778 Ubiquitination
K795 Ubiquitination
K808 Ubiquitination
K867 Ubiquitination
K874 Ubiquitination
T883 Phosphorylation
T1037 Phosphorylation P27708 (CAD)
S1038 Phosphorylation
S1044 Phosphorylation
S1061 Phosphorylation
S1069 Phosphorylation
S1073 Phosphorylation
S1112 Phosphorylation
K1119 Ubiquitination
K1127 Ubiquitination
K1177 Ubiquitination
K1206 Ubiquitination
K1211 Ubiquitination
S1220 Phosphorylation
S1222 Phosphorylation
K1228 Ubiquitination
K1262 Ubiquitination
S1267 Phosphorylation
K1301 Methylation
K1309 Ubiquitination
K1313 Ubiquitination
K1323 Ubiquitination
K1325 Ubiquitination
K1387 Ubiquitination
S1406 Phosphorylation P17612 (PRKACA)
S1407 Phosphorylation
K1411 Acetylation
K1411 Ubiquitination
S1423 Phosphorylation
K1431 Ubiquitination
K1434 Ubiquitination
K1450 Sumoylation
K1450 Ubiquitination
K1460 Ubiquitination
K1521 Ubiquitination
K1618 Ubiquitination
K1625 Ubiquitination
S1646 Phosphorylation
K1657 Ubiquitination
S1698 Phosphorylation
S1724 Phosphorylation
K1778 Ubiquitination
Y1794 Phosphorylation
Y1805 Phosphorylation
S1823 Phosphorylation
T1827 Phosphorylation
S1828 Phosphorylation
T1831 Phosphorylation
T1832 Phosphorylation
T1833 Phosphorylation
R1839 Methylation
H1856 Phosphorylation
S1859 Phosphorylation P23443 (RPS6KB1) , P17612 (PRKACA)
S1873 Phosphorylation
T1884 Phosphorylation
T1888 Phosphorylation
C1889 S-Nitrosylation
Y1890 Phosphorylation
S1900 Phosphorylation
T1906 Phosphorylation
T1914 Phosphorylation
S1920 Phosphorylation
S1938 Phosphorylation
K1953 Ubiquitination
K1961 Ubiquitination
K2036 Ubiquitination
Y2103 Phosphorylation
K2125 Ubiquitination
S2154 Phosphorylation
T2155 Phosphorylation
Y2158 Phosphorylation
K2178 Ubiquitination
Y2203 Phosphorylation
Y2212 Phosphorylation
T2220 Phosphorylation

翻译修饰 - P27708 作为激酶

Substrate Site Source
P27708 (CAD) T1037 Uniprot

研究背景

功能:

This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).

翻译修饰:

Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivity to feedback inhibition by UTP.

细胞定位:

Cytoplasm. Nucleus.
Note: Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homohexamer. Interacts with CIPC.

蛋白家族:

In the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.

研究领域

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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