Phospho-CAD (Ser1859) Antibody - #AF4415

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产品: 磷酸化 CAD (Ser1859) 抗体
货号: AF4415
描述: Rabbit polyclonal antibody to Phospho-CAD (Ser1859)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: P27708
RRID: AB_2844480

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 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-CAD (Ser1859) Antibody detects endogenous levels of CAD only when phosphorylated at Ser1859.
RRID:
AB_2844480
引用格式: Affinity Biosciences Cat# AF4415, RRID:AB_2844480.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Aspartate carbamoyltransferase; Aspartate transcarbamylase; CAD protein; CAD trifunctional protein; Carbamoyl phosphate synthetase 2; CPSase ATCase DHOase; Dihydroorotase; Glutamine dependent carbamoyl phosphate synthase; Multifunctional protein CAD;

抗原和靶标

免疫原:

A synthesized peptide derived from human CAD around the phosphorylation site of Ser1859.

基因/基因ID:
CAD(790)

研究领域

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Multi-omic analysis of precocious puberty girls: pathway changes and metabolite validation. Frontiers in endocrinology, 2024 (PubMed: 38487340) [IF=3.9]

Application: WB    Species: Rat    Sample:

Figure 9 Thymine administration delays the maturation of follicles and thickening of the uterine wall in PP model rats. (A) Serum levels of FSH, LH, E2, and leptin in two rat groups were detected by ELISA (n = 6). (B) HE staining of the uterus (×50, scale bar = 200 mm; ×200, scale bar = 100 mm) and ovary of two rat groups (×50, scale bar = 500 mm; ×200, scale bar = 100 mm) (n = 6). CL: corpus luteum; MF: mature follicle. (C) The thickening of the uterine wall and number of corpora lutea were calculated. (D) The mRNA expressions of FSHR and LHR in uterine tissues were detected by qRT-PCR. n = 3. (E) The expressions of GnRH in ovarian tissues were detected by immunohistochemical staining (n = 6). (F) The mRNA levels of GnRH, GnRHR, netrin-1, and KISS1 in hypothalamus tissues were detected by qRT-PCR (n = 3). (G) The protein expressions of p-CAD/CAD and CPS 2 were detected by Western blotting (n = 3).

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