Phospho-Cyclin E1 (Ser73) Antibody - #AF4413

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产品: 磷酸化 Cyclin E1 (Ser73) 抗体
货号: AF4413
描述: Rabbit polyclonal antibody to Phospho-Cyclin E1 (Ser73)
应用: WB IHC
文献验证: IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Chicken
蛋白号: P24864
RRID: AB_2844478

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Cyclin E1 (Ser73) Antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Ser73.
RRID:
AB_2844478
引用格式: Affinity Biosciences Cat# AF4413, RRID:AB_2844478.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CCNE; Ccne1; CCNE1_HUMAN; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1;

抗原和靶标

免疫原:

A synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Ser73.

基因/基因ID:
CCNE1(898)

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). AKAP95 regulates ubiquitination and degradation of cyclin Ds/Es, influencing the G1/S transition of lung cancer cells. Molecular carcinogenesis, 2024 (PubMed: 38923703) [IF=3.0]
2). Correlations of the expression of Cx43, SCFFBXW7, p-cyclin E1 (Ser73), p-cyclin E1 (Thr77) and p-cyclin E1 (Thr395) in colon cancer tissues. World journal of gastrointestinal oncology, 2025 (PubMed: 39817129) [IF=2.5]

Application: IHC    Species: human    Sample: colon cancer tissue

Figure 1 Expression of Cx43, Skp1-Cullin1-F-box (SCF)FBXW7, Phosphorylation of cyclin E1 (p-cyclin E1) (Ser73, Thr77, Thr395) in 38 colon cancer tissue cases. A: High expression of Cx43 in normal tissues; B: Low expression of Cx43 in colon cancer tissues; C: Medium expression of Cx43 in colon cancer tissues; D: High expression of Cx43 in colon cancer tissues; E: High expression of SCFFBXW7 in normal tissues; F: Low expression of SCFFBXW7 in colon cancer tissues; G: Medium expression of SCFFBXW7in colon cancer tissues; H: High expression of SCFFBXW7in colon cancer tissues; I: High expression of p-cyclin E1 (Ser73) in normal tissues; J: Low expression of p-cyclin E1(Ser73) in colon cancer tissues; K: High expression of p-cyclin E1 (Ser73) in colon cancer tissues; L: Negative expression of p-cyclin E1 (Thr77) in normal tissues; M: Negative expression of p-cyclin E1 (Thr77) in colon cancer tissues; N: High expression of p-cyclin E1(Thr395) in normal tissues; O: Medium expression of p-cyclin E1(Thr395) in colon cancer tissues; P: High expression of p-cyclin E1 (Thr395) in colon cancer tissues. Luan RG, Liu MD, Deng ZF, Lu CL, Yu ML, Zhang MY, Liu R, An R, Yao YL, Guo DB, Zhang YX, Zhao L. Correlations of the expression of Cx43, SCFFBXW7, p-cyclin E1 (Ser73), p-cyclin E1 (Thr77) and p-cyclin E1 (Thr395) in colon cancer tissues. World J Gastrointest Oncol 2025; 17(1): 98410 [PMID: 39817129 DOI: 10.4251/wjgo.v17.i1.98410]

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