Phospho-STK3 (Ser316) Antibody - #AF4314

Figure 2 High VEGF concentrations promote TAZ expression and nuclear localization in human TM cells. (A) Western blot analysis of TAZ protein and its phosphorylation in human TM cells at various VEGF concentrations. β-actin was the internal control. (B) Expression of TAZ and p-TAZ/TAZ was significantly upregulated at 10 and 30 ng/mL VEGF, whereas that of p-TAZ was not changed by VEGF exposure. (C) Quantification of p-TAZ abundance relative to TAZ. (D) Representative image demonstrating the subcellular localization of TAZ (green) and nuclei (blue) in human TM cells across the various VEGF concentrations. The Yellow arrows indicate TAZ localization in the nucleus. Asterisks (*) are used to show comparisons between the group of interest versus control cells; ** p < 0.01. Significance was determined using one-way ANOVA followed by Turkey’s multiple comparisons post-hoc test.