产品: p15 INK4b 抗体
货号: AF0230
描述: Rabbit polyclonal antibody to p15 INK4b
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Rabbit, Dog
分子量: 14kDa; 15kD(Calculated).
蛋白号: P42772
RRID: AB_2833405

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
p15 INK4b Antibody detects endogenous levels of total p15 INK4b.
RRID:
AB_2833405
引用格式: Affinity Biosciences Cat# AF0230, RRID:AB_2833405.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDK inhibitory protein; CDK4B Inhibitor; Cdkn2b; CDN2B_HUMAN; Cyclin dependent kinase 4 inhibitor B; Cyclin Dependent Kinase Inhibitor 2B; Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4; Cyclin dependent kinases 4 and 6 binding protein; Cyclin-dependent kinase 4 inhibitor B; INK4B; MTS 2; MTS-2; MTS2; Multiple tumor suppressor 2; Multiple Tumor Supressor 2; OTTHUMP00000021154; OTTHUMP00000021155; p14 CDK inhibitor; p14 INK4b; p14-INK4b; P15; p15 CDK inhibitor; p15 inhibits CDK4; p15 INK4b; p15-INK4b; p15INK4B; TP 15; TP15;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P42772 CDN2B_HUMAN:

Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.

描述:
p15-INK4B a potent inhibitor that nteracts strongly with CDK4 and CDK6. Potential effector of TGF-beta induced cell cycle arres
序列:
MREENKGMPSGGGSDEGLASAAARGLVEKVRQLLEAGADPNGVNRFGRRAIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEERGHRDVAGYLRTATGD

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Chicken
75
Pig
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P42772 作为底物

Site PTM Type Enzyme
C74 S-Nitrosylation
R101 Methylation

研究背景

功能:

Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.

细胞定位:

Cytoplasm.
Note: Also found in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.

亚基结构:

Heterodimer of CDKN2B with CDK4 or CDK6. Isoform 2 does not interact with CDK4 nor CDK6.

蛋白家族:

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). Sun L et al. Comprehensive Analysis of Cellular Senescence-Related Genes in Prognosis, Molecular Characterization and Immunotherapy of Hepatocellular Carcinoma. Biological Procedures Online 2022 Dec 19;24(24) (PubMed: 36536279) [IF=6.4]

Application: WB    Species: Human    Sample: HCC tissues and cells

Fig. 6 Western Blot showed the protein expression difference of 5 signature genes in HCC tissues and cells. A Differential protein expression of 5 signature genes in 8 pairs of HCC tissues and paraneoplastic tissues. B Differential protein expression of 5 signature genes in normal liver cell (7702) and HCC cells (LM3, 97H, HepG2, 7721 and Hu7)

Application: IHC    Species: Human    Sample: HCC tissues and cells

Fig. 7 Staining images of five signature genes in HCC tissues and paraneoplastic tissues. Relative optical density scores were used to compare the differences between the two groups. A CBX2; B CDKN2B; C ETS2; D HMGA1; E UBE2S

2). Chen D et al. ANRIL inhibits p15(INK4b) through the TGFβ1 signaling pathway in human esophageal squamous cell carcinoma. CELLULAR IMMUNOLOGY 2014 May-Jun;289(1-2):91-6 (PubMed: 24747824) [IF=4.3]

Application: WB    Species:    Sample:


3). Liu et al. INK4 cyclin-dependent kinase inhibitors as potential prognostic biomarkers and therapeutic targets in hepatocellular carcinoma. Bioscience Reports 2022 Jun 30; (PubMed: 35771229) [IF=4.0]

4). Jiang Y et al. Overexpression of SMYD3 in Ovarian Cancer is Associated with Ovarian Cancer Proliferation and Apoptosis via Methylating H3K4 and H4K20. Journal of Cancer 2019 Jul 8;10(17):4072-4084 (PubMed: 31417652) [IF=3.9]

Application: WB    Species: human    Sample: HEY and A2780 cells

Figure 5. |SMYD3 regulated ovarian cancer proliferation and apoptosis though SMYD3-H4K20me3-CDKN2A and SMYD3-H3K4me3-BIRC3, respectively. (A-B) The protein expression changes in the above 6 genes in the HEY and A2780 cell lines after SMYD3 knockdown in HEY and A2780 cells.

5). Wang et al. Comprehensive Analysis of INK4 Cyclin-Dependent Kinase Inhibitors in Hepatocellular Carcinoma.

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