Nrf2 Antibody - #AF7904

Figure 2 Nrf2 deficiency exacerbates sepsis-induced ALI and promotes inflammation in a CLP mouse model; n = 6 mice per group. (A) Histological analyses of the lung tissues in WT and Nrf2−/− mice in both the sham and CLP groups. (B) Representative lung injury scores in (A). (C,D) Western blot analyses of Nrf2 protein in WT and Nrf2−/− mice. (E,F) The expressions of inflammatory cytokines including IL-1β and TNF-α in BALFs were measured with ELISA. ** p < 0.01, *** p < 0.001.

FIGURE 4 Irisin upregulates the SIRT1/Nrf-2 pathway to inhibit LPS-induced ferroptosis. HK-2 cells were transfected with SIRT1 siRNA (si-Sirt1) or negative control (si-con) followed by exposure to LPS and irisin. (A) The expression of SIRT1 protein in cells transfected with SIRT1 siRNA (si-Sirt1) or negative control (si-con) was detected using Western blot to validate the knockdown of SIRT1. (B) Western blots and quantitative analyses of GPX4 and ACSL4 proteins in cells. (C) Western blots and quantitative analyses of SIRT1 and Nrf2 proteins in cells. (D) Cell viability using the CCK-8 kit. The levels of MDA (E) and GSH (F) in cells were analyzed using a corresponding kit. *p < 0.05 vs. si-con group, #p < 0.05 vs. si-con + LPS group, &p < 0.05 vs. si-con + LPS + Ir group. Data are presented as mean ± SD (n = 3).