CKII alpha Antibody - #AF7834

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产品: CKII alpha 抗体
货号: AF7834
描述: Rabbit polyclonal antibody to CKII alpha
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
蛋白号: P68400
RRID: AB_2844198

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
CKII alpha Antibody detects endogenous levels of total CKII alpha.
RRID:
AB_2844198
引用格式: Affinity Biosciences Cat# AF7834, RRID:AB_2844198.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Casein kinase 2 alpha 1 polypeptide; Casein kinase II alpha 1; Casein kinase II alpha 1 subunit; Casein kinase II alpha subunit; Casein kinase II subunit alpha; CK II alpha; CK II; CK2 alpha; CK2 catalytic subunit alpha; CK2A1; CKII; CKIIalpha; CSK21_HUMAN; CSNK2A1;

抗原和靶标

免疫原:

A synthesized peptide derived from human CKII alpha, corresponding to a region within C-terminal amino acids.

基因/基因ID:
CSNK2A1(1457)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

文献引用

1). Ginsenoside Rh1, a novel casein kinase II subunit alpha (CK2α) inhibitor, retards metastasis via disrupting HHEX/CCL20 signaling cascade involved in tumor cell extravasation across endothelial barrier. Pharmacological research, 2023 (PubMed: 37944834) [IF=9.1]

Application: WB    Species: Mouse    Sample:

Fig. 8. Rh1 inhibited the enzyme activity of CK2α that curbs the nuclear translocation of HHEX. (A) The expression of CSNK2A1 gene in the 96 paired normal and BC samples on the basis of the TCGA database. (B) Overall survival rates of 80 months between CSNK2A1 high and low expression groups in all BC patients based on the TCGA database. (C) Western blot of CK2α in the indicated groups. (D) Quantification of protein expression level of CK2α (n = 3). (E) Quantification of the secretion of CCL20 (n = 4). (F) Quantification for the enzyme activity of CK2α in the indicated groups (n = 6). (G) Docking analysis of Rh1 with CK2α (PDB: 3NGA). (H) Interaction pattern of binding sites of CK2α for Rh1. (I) MST for determining the bind affinity of Rh1 to purified protein CK2α. (J) The E0771 BC cells were incubated with Rh1, followed by that the cells were collected and subjected to temperature-dependent CETSA assay for CK2α. (K) Quantification of the protein in (H) (n = 3). **P 

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