产品: | 磷酸化 NF2 / Merlin (Ser518) 抗体 |
货号: | AF3271 |
描述: | Rabbit polyclonal antibody to Phospho-NF2 / Merlin (Ser518) |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat |
预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 70kDa; 70kD(Calculated). |
蛋白号: | P35240 |
RRID: | AB_2834697 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3271, RRID:AB_2834697.
展开/折叠
ACN; BANF; Bilateral acoustic neuroma; MERL_HUMAN; Merlin; Moesin ezrin radixin like protein; Moesin ezrin radizin like; Moesin-ezrin-radixin-like protein; Neurofibromatosis 2; Neurofibromatosis type 2; Neurofibromatosis2; Neurofibromin 2; Neurofibromin-2; Neurofibromin2; NF 2; Nf2; SCH; Schwannomerlin; Schwannomin;
抗原和靶标
Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
- P35240 MERL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKIYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P35240 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S10 | Phosphorylation | P31749 (AKT1) , P17612 (PRKACA) | Uniprot |
S13 | Phosphorylation | Uniprot | |
K15 | Sumoylation | Uniprot | |
K76 | Sumoylation | Uniprot | |
K80 | Ubiquitination | Uniprot | |
K88 | Ubiquitination | Uniprot | |
K159 | Ubiquitination | Uniprot | |
K171 | Ubiquitination | Uniprot | |
T230 | Phosphorylation | P31749 (AKT1) | Uniprot |
K253 | Ubiquitination | Uniprot | |
K269 | Ubiquitination | Uniprot | |
K279 | Acetylation | Uniprot | |
K289 | Ubiquitination | Uniprot | |
S315 | Phosphorylation | P31749 (AKT1) | Uniprot |
K322 | Ubiquitination | Uniprot | |
R351 | Methylation | Uniprot | |
R353 | Methylation | Uniprot | |
K364 | Ubiquitination | Uniprot | |
K387 | Ubiquitination | Uniprot | |
K396 | Ubiquitination | Uniprot | |
K401 | Ubiquitination | Uniprot | |
T415 | Phosphorylation | Uniprot | |
T419 | Phosphorylation | Uniprot | |
K439 | Ubiquitination | Uniprot | |
K449 | Ubiquitination | Uniprot | |
K455 | Ubiquitination | Uniprot | |
T477 | Phosphorylation | Uniprot | |
S518 | Phosphorylation | Q13153 (PAK1) , Q13177 (PAK2) , P17612 (PRKACA) | Uniprot |
K533 | Ubiquitination | Uniprot | |
K550 | Sumoylation | Uniprot | |
K550 | Ubiquitination | Uniprot | |
R568 | Methylation | Uniprot | |
S572 | Phosphorylation | Uniprot | |
T576 | Phosphorylation | Uniprot | |
K579 | Ubiquitination | Uniprot | |
T581 | Phosphorylation | Uniprot | |
S584 | Phosphorylation | Uniprot | |
K586 | Ubiquitination | Uniprot | |
S587 | Phosphorylation | Uniprot |
研究背景
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail. The dephosphorylation of Ser-518 favors the interaction with NOP53.
Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation.
Cell projection>Filopodium membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Nucleus.
Note: In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with DCAF1 in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.
Cytoplasm>Perinuclear region. Cytoplasmic granule.
Note: Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 7 is absent from ruffling membranes and filopodia.
Cytoplasm>Perinuclear region. Cytoplasmic granule.
Note: Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia.
Nucleus. Cell projection>Filopodium membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Perinuclear region. Cytoplasmic granule. Cytoplasm>Cytoskeleton.
Note: In a fibroblastic cell line, isoform 10 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia.
Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
Interacts with SLC9A3R1, HGS and AGAP2. Interacts with LAYN (By similarity). Interacts with SGSM3. Interacts (via FERM domain) with MPP1. Interacts with WWC1. Interacts with the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. The unphosphorylated form interacts (via FERM domain) with VPRBP/DCAF1. Interacts (via FERM domain) with NOP53; the interaction is direct. Interacts with SCHIP1; the interaction is direct.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.