KIF20A Antibody - #AF7664

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产品: KIF20A 抗体
货号: AF7664
描述: Rabbit polyclonal antibody to KIF20A
应用: WB IF/ICC
文献验证: WB
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: O95235
RRID: AB_2844028

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
KIF20A Antibody detects endogenous levels of total KIF20A.
RRID:
AB_2844028
引用格式: Affinity Biosciences Cat# AF7664, RRID:AB_2844028.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AA415432; FLJ21151; GG10 2; GG10_2; KI20A_HUMAN; KIF20A; kinesin family member 20A; Kinesin like protein KIF20A; Kinesin-like protein 174; Kinesin-like protein KIF20A; mitotic kinesin like protein 2; Mitotic kinesin-like protein 2; MKlp2; OTTHUMP00000159481; RAB6 interacting, kinesin like (rabkinesin6); Rab6-interacting kinesin-like protein; RAB6-INTERACTING PROTEIN, KINESIN-LIKE; RAB6KIFL; Rabkinesin 6; Rabkinesin-6;

抗原和靶标

免疫原:

A synthesized peptide derived from human KIF20A, corresponding to a region within C-terminal amino acids.

基因/基因ID:
KIF20A(10112)

文献引用

1). KIF20A is associated with clinical prognosis and synergistic effect of gemcitabine combined with ferroptosis inducer in lung adenocarcinoma. Frontiers in pharmacology, 2022 (PubMed: 36225575) [IF=5.6]

Application: WB    Species: human    Sample: PC9 and A549 cell

FIGURE 7. KIF20A was highly expressed in LUAD cells and regulated the combined effect of GEM and IKE. (A) IHC staining of KIF20A protein in LUAD tissues was analyzed based on the HPA database. (B) Western blot analysis was used to detect KIF20A protein levels in human bronchial epithelial-like cells (16HBE) and LUAD cell lines (H358, PC9, HCC8217, H1299, A549). (C) RT-qPCR for detection of KIF20A mRNA levels in 16HBE and LUAD cell lines. (D) Western blot analysis for detecting knockdown of KIF20A in PC9 and A549. (E) Lipid ROS production was measured by flow cytometry using C11-BODIPY. A549/PC9 cells were treated with IKE for 24 h (F) CCK8 assay to detect the IC50 value of GEM in A549/PC9 after KIF20A knockdown. (G) Colony-forming Assay to assess the effect of KIF20A knockdown on the combined IKE and GEM. n = 3. LUAD: lung adenocarcinoma; GEM: Gemcitabine; IHC: Immunohistochemical; RT-qPCR: Real-Time Quantitative PCR; ROS: reactive oxygen species; IKE: Imidazole Ketone Erastin; IC50: half maximal inhibitory concentration; CCK8 assay: Cell Counting Kit-8 assay; G + I: Gemcitabine + IKE. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns p > 0.05.

Application: IHC    Species: human    Sample: PC9 and A549 cell

FIGURE 7. KIF20A was highly expressed in LUAD cells and regulated the combined effect of GEM and IKE. (A) IHC staining of KIF20A protein in LUAD tissues was analyzed based on the HPA database. (B) Western blot analysis was used to detect KIF20A protein levels in human bronchial epithelial-like cells (16HBE) and LUAD cell lines (H358, PC9, HCC8217, H1299, A549). (C) RT-qPCR for detection of KIF20A mRNA levels in 16HBE and LUAD cell lines. (D) Western blot analysis for detecting knockdown of KIF20A in PC9 and A549. (E) Lipid ROS production was measured by flow cytometry using C11-BODIPY. A549/PC9 cells were treated with IKE for 24 h (F) CCK8 assay to detect the IC50 value of GEM in A549/PC9 after KIF20A knockdown. (G) Colony-forming Assay to assess the effect of KIF20A knockdown on the combined IKE and GEM. n = 3. LUAD: lung adenocarcinoma; GEM: Gemcitabine; IHC: Immunohistochemical; RT-qPCR: Real-Time Quantitative PCR; ROS: reactive oxygen species; IKE: Imidazole Ketone Erastin; IC50: half maximal inhibitory concentration; CCK8 assay: Cell Counting Kit-8 assay; G + I: Gemcitabine + IKE. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns p > 0.05.
2). KIF20A Promotes CRC Progression and the Warburg Effect through the C-Myc/HIF-1α Axis. Protein and peptide letters, 2024 (PubMed: 38037834) [IF=1.0]

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