(3)
(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3258, RRID:AB_2834684.
展开/折叠
CDC 25; Cdc 25C; CDC25; CDC25C; Cell division cycle 25 homolog C; Cell division cycle 25C; Cell division cycle 25C protein; Dual specificity phosphatase Cdc25C; M phase inducer phosphatase 3; M-phase inducer phosphatase 3; Mitosis inducer CDC25; MPIP3; MPIP3_HUMAN; Phosphotyrosine phosphatase; PPP1R60; protein phosphatase 1, regulatory subunit 60;
抗原和靶标
- P30307 MPIP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKRCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLMKCSPAQLLCSTPNGLDRGHRKRDAMCSSSANKENDNGNLVDSEMKYLGSPITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITITQMLEEDSNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLKKPIVPLDTQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIALLVKDMSP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P30307 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K52 | Ubiquitination | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| T67 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K69 | Ubiquitination | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S129 | Phosphorylation | Uniprot | |
| T130 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S146 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| Y165 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | P45984 (MAPK9) , P53779 (MAPK10) , P45983 (MAPK8) , P06493 (CDK1) | Uniprot |
| T171 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Q9H4B4 (PLK3) | Uniprot |
| S198 | Phosphorylation | Q9H4B4 (PLK3) , PR:P53350 (hPLK1) , P53350 (PLK1) | Uniprot |
| S207 | Phosphorylation | P53778 (MAPK12) | Uniprot |
| S209 | Phosphorylation | Uniprot | |
| Y212 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| S216 | Phosphorylation | P27361 (MAPK3) , Q13237 (PRKG2) , Q13535 (ATR) , Q683Z8 (CHK2) , P28482 (MAPK1) , Q16539 (MAPK14) , P49137 (MAPKAPK2) , Q16512 (PKN1) , O14757 (CHEK1) , Q9H4B4 (PLK3) , P27448 (MARK3) , P43405 (SYK) , Q8TDC3 (BRSK1) , O96017 (CHEK2) | Uniprot |
| K233 | Ubiquitination | Uniprot | |
| T236 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K240 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| S247 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| K251 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| Y401 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| Y416 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| S472 | Phosphorylation | Uniprot |
研究背景
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216. However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.
Nucleus.
Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated on Ser-129 and/or Thr-130, interacts with PLK1.
(Microbial infection) Interacts with HIV-1 Vpr; this interaction inactivates CDC25C phosphatase activity.
Belongs to the MPI phosphatase family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
文献引用
Application: WB Species: Human Sample: NSCLC cell
Application: WB Species: human Sample: NSCLC cells
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