PAX7 Antibody - #AF7584

Fig. 3 Results of protein expression levels of MyoD1, Pax7, Myf5 and MyoG. (A) Western blot of MyoG, Myf5, Pax7, MyoD1, and GAPDH. (B, C) Quantitative results of protein expression in rectus femoris muscle of each group on 10 and 20 days of traction treatment, respectively (n = 8). *P 

Fig. 2.Bi-phasic effect of gelatin in activating quiescent satellite cells (SCs) and myogenesis during muscle regeneration. (A) Immunohistochemical (IHC) analysis of Pax7+ SCs (green) in TA muscle stained by laminin (red) at 7 D.P.I. Arrows point to Pax7+ nuclei. Scale bar: 50 μm. (B) The percentage of myofibers containing Pax7+ SCs (n=6). (C) IHC analysis of MyoG+ cells (green) in damaged TA muscle stained by laminin (red) at 7 D.P.I. Arrows point to MyoG+ nuclei. Scale bar: 50 μm. (D) The percentage of myofibers containing MyoG+ cells (n=6). (E,F) Western blots (E) and quantification (F) of MyoD and MyoG in damaged TA muscles at 14 D.P.I. (n=3). GAPDH is used as a loading control. Significance was determined by unpaired two-tailed Student's t-test with Welch's correction.

Figure 2 Generation of Tmem30a conditional knockout (cKO) mice A: Breeding strategy of cKO mice by crossing Tmem30aloxP/loxP with Pax7CreER mice. B: Genotyping of mice by PCR and agarose gel electrophoresis. C: Axis of tamoxifen intraperitoneal injection, BaCl2 injection, and TA muscle harvesting in mice. D: In 7 dpi cKO mice, expression of Pax7CreER was monitored by ROSA-tdTomato reporter (red). SCs were labeled with Pax7 antibody (green). Nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. E: TMEM30A protein from 7 dpi WT and cKO mice was labeled with TMEM30A antibody (green) and DAPI (blue). Scale bar: 50 μm. F, G: Immunoblot analysis of TMEM30A protein expression in 7 dpi WT and cKO mice. Relative protein band density was calculated by ImageJ. Data are mean±SEM. n=3 in each group. Significance was calculated by two-tailed Student’s t-test. *: P<0.05.

Figure 5