osterix Antibody - #AF7580

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产品: osterix 抗体
货号: AF7580
描述: Rabbit polyclonal antibody to osterix
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 46kDa, 37 kDa; 45kD(Calculated).
蛋白号: Q8TDD2
RRID: AB_2843944

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Rabbit(%), Dog(%), Xenopus(%)
克隆:
Polyclonal
特异性:
osterix Antibody detects endogenous levels of total osterix.
RRID:
AB_2843944
引用格式: Affinity Biosciences Cat# AF7580, RRID:AB_2843944.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

MGC126598; Osterix; Osx; Sp 7; SP7; Sp7 transcription factor; SP7_HUMAN; Transcription factor Sp7; Zinc finger protein osterix;

抗原和靶标

免疫原:
Uniprot:

Q8TDD2(SP7_HUMAN) >>Visit HPA database.

基因/基因ID:
SP7(121340)
表达:
Q8TDD2 SP7_HUMAN:

Restricted to bone-derived cell.

序列:
MASSLLEEEVHYGSSPLAMLTAACSKFGGSSPLRDSTTLGKAGTKKPYSVGSDLSASKTMGDAYPAPFTSTNGLLSPAGSPPAPTSGYANDYPPFSHSFPGPTGTQDPGLLVPKGHSSSDCLPSVYTSLDMTHPYGSWYKAGIHAGISPGPGNTPTPWWDMHPGGNWLGGGQGQGDGLQGTLPTGPAQPPLNPQLPTYPSDFAPLNPAPYPAPHLLQPGPQHVLPQDVYKPKAVGNSGQLEGSGGAKPPRGASTGGSGGYGGSGAGRSSCDCPNCQELERLGAAAAGLRKKPIHSCHIPGCGKVYGKASHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELERHVRTHTREKKFTCLLCSKRFTRSDHLSKHQRTHGEPGPGPPPSGPKELGEGRSTGEEEASQTPRPSASPATPEKAPGGSPEQSNLLEI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).

Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.

翻译修饰:

Ubiquitination at leads to proteasomal degradation. SP7 is a short-live protein with an endogenous half-life of approximately 12 hours.

Propionylated. Depropionylation at Lys-371 by SIRT7 activates transcription factor activity and positively regulates bone formation by osteoblasts.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Restricted to bone-derived cell.

亚基结构:

Interacts with RIOX1; the interaction is direct and inhibits transcription activator activity.

蛋白家族:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the Sp1 C2H2-type zinc-finger protein family.

文献引用

1). MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm. Cell Death & Differentiation, 2021 (PubMed: 33664480) [IF=13.7]

Application: WB    Species: Human    Sample: MACF1-KD cells

Fig. 1 MACF1 knockdown inhibits osteoblast differentiation and alters osteoblast differentiation transcriptome dynamics. A ALP activity of Control and MACF1-KD cells was detected by ALP staining. B The expression of ALP and Runx2 examined by real-time PCR during osteoblastic differentiation in Control and MACF1-KD cells. Data represent mean value ± SD (n = 3). (**P 
2). Metformin protects against vascular calcification through the selective degradation of Runx2 by the p62 autophagy receptor. Journal of Cellular Physiology, 2022 (PubMed: 36166694) [IF=4.5]

Application: WB    Species: Mouse    Sample: VSMCs

FIGURE 1Metformin activates AMPK and calcification inVSMCs. VSMCs were cultured with high phosphate (3 mM Pi) and metformin (met)for 7 days. (a) Calcium content (mg/mg protein) of cells treated with metformin (0.5−1.5 mM), (n= 3). (b) Representative alizarin red images ofcells treated with metformin (1 mM). (c) Representative immunoblots (d) immunoblot quantification showing the effect of metformin (1 mM) onexpression of osteogenic markers osterix, osteocalcin, and bone sialoprotein compared withβ‐actin (n= 3). (e) Representative immunoblots and(f) immunoblot quantification showing the effect of metformin (1 mM) on the phosphorylation of AMPK compared with total AMPK (n= 4).AMPK, adenosine monophosphate‐activated protein kinase; VSMCs, vascular smooth muscle cells.PHADWALET AL.|4307

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